Scalable Additive Construction of Arrayed Microstructures with Encoded Properties for Bioimaging.

Scalable Additive Construction of Arrayed Microstructures with Encoded Properties for Bioimaging.
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DOI:
10.3390/mi13091392
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发表时间:
2022-08-25
期刊:
影响因子:
3.4
通讯作者:
Allbritton NL
Allbritton NL
中科院分区:
工程技术3区
文献类型:
--
作者:
DiSalvo M;Cortés-Llanos B;LaBelle CA;Murdoch DM;Allbritton NL

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微阵列是分析仪器的重要组成部分。使用复合材料和结构,微阵列的元件可以被赋予额外的功能和编码,但传统的微加工方法对制造、设计和可扩展性提出了巨大的障碍。在这项工作中,报道了一种免工具技术,可以批量增材制造微成型、复合和阵列微结构。该方法仅需要兼容的载液即可将材料沉积到具有一定形貌的基板上。利用这种基本制造方法的排列来控制微结构内沉积材料的体积和位置。作为概念验证,构建了细胞微载体阵列来展示复合微结构的一系列设计、成分、功能和应用。这种方法预计将能够制造复杂的复合生物和合成微量元素,用于生物传感、细胞分析和生化筛选。
Microarrays are essential components of analytical instruments. The elements of microarrays may be imbued with additional functionalities and encodings using composite materials and structures, but traditional microfabrication methods present substantial barriers to fabrication, design, and scalability. In this work, a tool-free technique was reported to additively batch-construct micromolded, composite, and arrayed microstructures. The method required only a compatible carrier fluid to deposit a material onto a substrate with some topography. Permutations of this basic fabrication approach were leveraged to gain control over the volumes and positions of deposited materials within the microstructures. As a proof of concept, cell micro-carrier arrays were constructed to demonstrate a range of designs, compositions, functionalities, and applications for composite microstructures. This approach is envisioned to enable the fabrication of complex composite biological and synthetic microelements for biosensing, cellular analysis, and biochemical screening.
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