Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization.
Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization.
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由肽核酸荧光原位杂交在两个圈养的鲨鱼中检测到的野生菌菌引起的生殖器分枝杆菌病引起的。
DOI：
10.1111/jfd.13716
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发表时间：
2023-01
影响因子：
2.5
通讯作者：
Wada, Shinpei
中科院分区：
文献类型：
--
作者：
Inohana, Mari;Komine, Takeshi;Tanaka, Yoshiaki;Kurata, Osamu;Wada, Shinpei
海分枝杆菌是一种流行的非结核分枝杆菌（NTM）感染硬骨鱼。相反，对板鳃类中的分枝杆菌病知之甚少，并且从未报道过该亚类中的海洋分枝杆菌感染。本研究通过对同一水族箱中的双髻鲨Sphyrna lewini和日本牛头鲨Heterodontus lewini的分子生物学分析，研究了该分支杆菌的组织病理学特征和定位。我们使用分子分析检测由海洋分枝杆菌感染引起的生殖器分枝杆菌病，包括靶向60 kDa热休克蛋白基因（hsp 65）的聚合酶链反应（PCR）和DNA测序，以及靶向16 S rRNA基因的肽核酸荧光原位杂交（PNA-FISH）。两条鲨鱼的生殖腺结缔组织中均出现肉芽肿，但没有中央坏死或周围纤维囊，这与硬骨鱼中常见的典型分枝杆菌肉芽肿不同。本研究表明板鳃类动物可以作为海洋微囊藻的水生宿主。由于M. marinum是一种代表性的水生NTM和潜在的人畜共患病原体，需要谨慎和深入的研究，以克服缺乏数据的NTM和水环境之间的关系与软骨鱼类的这个亚类。
Mycobacterium marinum is a prevalent nontuberculous mycobacterium (NTM)‐infecting teleosts. Conversely, little is known about mycobacteriosis in elasmobranchs, and M. marinum infection has never been reported from the subclass. This study investigated the histopathological characteristics and localization of this mycobacterium through molecular analysis of two captive sharks, a scalloped hammerhead Sphyrna lewini and a Japanese bullhead shark Heterodontus japonicus, exhibited in the same aquarium tank. We detected genital mycobacteriosis caused by M. marinum infection using molecular analyses, including polymerase chain reaction (PCR) and DNA sequencing targeting the 60 kDa heat‐shock protein gene (hsp65), and peptide nucleic acid–fluorescence in situ hybridization (PNA‐FISH) targeting the 16S rRNA gene. Both sharks showed granulomas in connective tissues of the gonads without central necrosis or surrounding fibrous capsules, which is unlike the typical mycobacterial granulomas seen in teleosts. This study reveals that elasmobranchs can be aquatic hosts of M. marinum. Because M. marinum is a representative waterborne NTM and a potential zoonotic agent, cautious and intensive research is needed to overcome a lack of data on the relationship between NTM and the aquatic environment in association with this subclass of Chondrichthyes.
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影响因子：
9.4
作者：
Phillips, R;Horsfield, C;Wansbrough-Jones, M
通讯作者：
Wansbrough-Jones, M
DOI：
10.1099/ijs.0.63343-0
发表时间：
2005-05-01
影响因子：
2.8
作者：
Rhodes, MW;Kator, H;Ottinger, CA
通讯作者：
Ottinger, CA
影响因子：
2.5
作者：
Anderson, E. T.;Frasca, S., Jr.;Harms, C. A.
通讯作者：
Harms, C. A.
影响因子：
2.5
作者：
Novotny, L.;Halouzka, R.;Pavlik, I.
通讯作者：
Pavlik, I.
影响因子：
4.2
作者：
Oh TH;Kim UJ;Kang SJ;Jang HC;Park KH;Jung SI;Ahn JH
通讯作者：
Ahn JH