Cardiac arrhythmia and injury induced in rats by burst and pulsed mode ultrasound with a gas body contrast agent.
Cardiac arrhythmia and injury induced in rats by burst and pulsed mode ultrasound with a gas body contrast agent.
复制标题
DOI：
10.7863/jum.2009.28.11.1519
复制
发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
Lucchesi BR
中科院分区：
文献类型：
--
作者：
Miller DL;Dou C;Lucchesi BR
心肌超声造影剂和burst模式（物理治疗）超声在较低的峰值稀疏压力幅值（prpa）下也报道了心电图（ECG）信号中的过早复合体（PCs）。对比超声心动图显示，不可逆损伤的心肌细胞与心律失常有关。目的是确定心肌细胞损伤是否与低prpa时突发模式诱导的PCs有关。麻醉后的大鼠在水浴中接受1.5 MHz聚焦超声。静脉注射Evans蓝染料对损伤心肌细胞进行染色，静脉注射Definity超声造影剂。连续爆发模式模拟物理治疗超声。在收缩末期1:4触发间歇性的2毫秒脉冲，或模拟诊断超声的脉冲包络。在心电记录上观察pc，在冷冻切片上计数染色的心肌细胞。连续爆发模式产生可变的pc和0.3 MPa以上PRPA染色细胞。触发脉冲在0.3 MPa以上和脉冲包膜在1.2 MPa以上时，心肌细胞染色和pc的产生均有统计学意义（P<0.01）。不可逆心肌细胞损伤与突发模式下PCs的发生有关，并且发生在比脉冲超声低得多的prpa下。
Premature complexes (PCs) in the electrocardiogram (ECG) signal have been reported for myocardial contrast echocardiography and also for burst mode (physical therapy) ultrasound with gas body contrast agent at lower peak rarefactional pressure amplitudes (PRPAs). For contrast echocardiography, irreversibly injured cardiomyocytes have been associated with the arrhythmia. The objective was to determine if cardiomyocyte injury is associated with the PCs induced by the burst mode at lower PRPAs. Anesthetized rats were exposed to focused 1.5 MHz ultrasound in a water bath. Evans blue dye was injected IP to stain injured cardiomyocytes and Definity ultrasound contrast agent was infused IV. Continuous burst mode simulated physical therapy ultrasound. Intermittent 2 ms bursts, or envelopes of pulses simulating diagnostic ultrasound, were triggered 1:4 at end systole. PCs were observed on ECG recordings and stained cardiomyocytes were counted in frozen sections. The continuous burst mode produced variable PCs and stained cells above 0.3 MPa PRPA. The triggered bursts above 0.3 MPa and pulse envelopes above 1.2 MPa produced statistically significant (P<0.01) PCs and stained cardiomyocytes. Irreversible cardiomyocyte injury was associated with the development of PCs for burst mode and occurred at substantially lower PRPAs than for pulsed ultrasound.
登录
查看更多内容
影响因子：
2.3
作者：
Chapman, S;Windle, J;Porter, TR
通讯作者：
Porter, TR
影响因子：
19.7
作者：
Miller, DL;Li, P;Armstrong, WF
通讯作者：
Armstrong, WF
影响因子：
2.4
作者：
Miller, Douglas L.;Dou, Chunyan;Wiggins, Roger C.
通讯作者：
Wiggins, Roger C.
DOI：
10.1067/mje.2001.112750
发表时间：
2001-06-01
影响因子：
6.5
作者：
Porter, TR;Everbach, C;Xie, E
通讯作者：
Xie, E
影响因子：
2.3
作者：
Zachary, JF;Hartleben, SA;O'Brien, WD
通讯作者：
O'Brien, WD