dHb9 expressing larval motor neurons persist through metamorphosis to innervate adult-specific muscle targets and function in Drosophila eclosion.
dHb9 expressing larval motor neurons persist through metamorphosis to innervate adult-specific muscle targets and function in Drosophila eclosion.
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DOI：
10.1002/dneu.22400
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发表时间：
2016-12
影响因子：
3
通讯作者：
Fernandes, Joyce
中科院分区：
文献类型：
--
作者：
Banerjee, Soumya;Toral, Marcus;Siefert, Matthew;Conway, David;Dorr, Meredith;Fernandes, Joyce
果蝇幼虫的神经系统在蜕变过程中发生了根本性的重构，以产生成虫特有的神经回路和行为。新神经元的形成，幼虫神经元的死亡，以及那些持续存在的神经元的重塑，这些神经元共同塑造了成年神经系统。在这里，我们研究了一个子集的幼虫运动神经元在这个重组过程中的命运。我们使用dHb9报告基因，结合FLP/FRT系统，结合相对细胞体（CB）位置、轴突位置和肌肉靶点，分别鉴定了幼虫到成虫转变过程中的腹部运动神经元。我们发现一些表达dHb9的运动神经元的节段特异性细胞死亡发生在整个蜕变期，并持续到羽化后。然而，许多表达dHb9>GFP的神经元存在于A1和A2这两个具有羽化所需的节段特异性肌肉的前半节段，而较小比例的神经元也存在于A2 - a5。与这些神经元的功能要求一致，在蛹期消融它们会在成年羽化中产生缺陷。在成人中，在执行羽化行为后，发现其中一些神经元的NMJs被拆除，其肌肉目标退化。我们的研究表明，一些幼虫运动神经元在成体运动过程中具有重要的连续性，并揭示了运动神经元重塑和可塑性的多个方面是成体运动行为的必要条件。
The Drosophila larval nervous system is radically restructured during metamorphosis to produce adult specific neural circuits and behaviors. Genesis of new neurons, death of larval neurons and remodeling of those neurons that persistent collectively act to shape the adult nervous system. Here we examine the fate of a subset of larval motor neurons during this restructuring process. We used a dHb9 reporter, in combination with the FLP/FRT system to individually identify abdominal motor neurons in the larval to adult transition using a combination of relative cell body (CB) location, axonal position and muscle targets. We found that segment specific cell death of some dHb9 expressing motor neurons occurs throughout the metamorphosis period and continues into the post-eclosion period. Many dHb9>GFP expressing neurons however persist in the two anterior hemisegments, A1 and A2, which have segment specific muscles required for eclosion while a smaller proportion also persist in A2–A5. Consistent with a functional requirement for these neurons, ablating them during the pupal period produces defects in adult eclosion. In adults, subsequent to the execution of eclosion behaviors, the NMJs of some of these neurons were found to be dismantled and their muscle targets degenerate. Our studies demonstrate a critical continuity of some larval motor neurons into adults and reveal that multiple aspects of motor neuron remodeling and plasticity that are essential adult motor behaviors.
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影响因子：
16.2
作者：
Broihier, HT;Skeath, JB
通讯作者：
Skeath, JB
影响因子：
11.4
作者：
HOOPER, JE
通讯作者：
HOOPER, JE
DOI：
10.1002/neu.20279
发表时间：
2006-09-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Hebbar, Sarita;Hall, Rachel E.;Fernandes, Joyce J.
通讯作者：
Fernandes, Joyce J.
影响因子：
2.7
作者：
Hoang, B;Chiba, A
通讯作者：
Chiba, A
影响因子：
2.7
作者：
Kim, Michael D.;Wen, Yuhui;Jan, Yuh-Nung
通讯作者：
Jan, Yuh-Nung