Imaging mass spectrometry technology and application on ganglioside study; visualization of age-dependent accumulation of C20-ganglioside molecular species in the mouse hippocampus.
Imaging mass spectrometry technology and application on ganglioside study; visualization of age-dependent accumulation of C20-ganglioside molecular species in the mouse hippocampus.
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DOI：
10.1371/journal.pone.0003232
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发表时间：
2008-09-18
期刊：
影响因子：
3.7
通讯作者：
Setou M
中科院分区：
文献类型：
--
作者：
Sugiura Y;Shimma S;Konishi Y;Yamada MK;Setou M
神经节苷脂在中枢神经系统（CNS）中特别丰富，并被认为在记忆形成、神经突发生、突触传递和其他神经功能中发挥重要作用。虽然神经节苷脂的几种分子种类已被表征，并阐明了它们各自的功能，但它们在CNS中的差异分布还不清楚。特别是，不同的分子种类是否在大脑中表现出不同的分布模式仍不清楚。我们报告的神经节苷脂分子种类的独特和特征的分布，所揭示的成像质谱（IMS）。该技术可以通过确定质荷比的差异以及通过串联质谱法的结构分析来区分从寡糖和神经酰胺结构中产生的分子种类。CNS中的神经节苷脂的特征在于神经酰胺部分中的长链碱基（LCB）的结构。主要神经节苷脂种类的LCB具有18或20个碳（即，C18-或C20-鞘氨醇）;我们发现这两种类型的神经节苷脂在小鼠脑中的分布是不同的。而C18-物种广泛分布在整个额叶脑，C20-物种选择性地定位沿着内鼻-海马突起，特别是在分子层（ML）的齿状回（DG）。我们揭示了发展和衰老相关的积累的C-20物种的ML-DG。因此，可以认为LCB链长度的这种脑区域特异性调节对于CNS细胞中的独特功能特别重要。
Gangliosides are particularly abundant in the central nervous system (CNS) and thought to play important roles in memory formation, neuritogenesis, synaptic transmission, and other neural functions. Although several molecular species of gangliosides have been characterized and their individual functions elucidated, their differential distribution in the CNS are not well understood. In particular, whether the different molecular species show different distribution patterns in the brain remains unclear. We report the distinct and characteristic distributions of ganglioside molecular species, as revealed by imaging mass spectrometry (IMS). This technique can discriminate the molecular species, raised from both oligosaccharide and ceramide structure by determining the difference of the mass-to-charge ratio, and structural analysis by tandem mass spectrometry. Gangliosides in the CNS are characterized by the structure of the long-chain base (LCB) in the ceramide moiety. The LCB of the main ganglioside species has either 18 or 20 carbons (i.e., C18- or C20-sphingosine); we found that these 2 types of gangliosides are differentially distributed in the mouse brain. While the C18-species was widely distributed throughout the frontal brain, the C20-species selectively localized along the entorhinal-hippocampus projections, especially in the molecular layer (ML) of the dentate gyrus (DG). We revealed development- and aging-related accumulation of the C-20 species in the ML-DG. Thus it is possible to consider that this brain-region specific regulation of LCB chain length is particularly important for the distinct function in cells of CNS.
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影响因子：
2.7
作者：
GLASIER, MM;SUTTON, RL;STEIN, DG
通讯作者：
STEIN, DG
影响因子：
2.9
作者：
KOTANI, M;TERASHIMA, T;TAI, T
通讯作者：
TAI, T
影响因子：
4.1
作者：
MASSERINI, M;PALESTINI, P;TETTAMANTI, G
通讯作者：
TETTAMANTI, G
影响因子：
2.7
作者：
RAHMANN, H
通讯作者：
RAHMANN, H
影响因子：
4.7
作者：
PALESTINI, P;MASSERINI, M;TETTAMANTI, G
通讯作者：
TETTAMANTI, G