Heterogeneity of Rat Neurofilament Polypeptides Revealed by a Monoclonal Antibody
Heterogeneity of Rat Neurofilament Polypeptides Revealed by a Monoclonal Antibody
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单克隆抗体揭示大鼠神经丝多肽的异质性
DOI：
10.1111/j.1471-4159.1986.tb13023.x
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发表时间：
1986
影响因子：
4.7
通讯作者：
S. Schiaffino
中科院分区：
文献类型：
--
作者：
M. Vitadello;C. Triban;M. Fabris;A. Gorio;S. Schiaffino
摘要：从用新生大鼠大脑的粗神经丝制剂免疫的小鼠中获得的单克隆抗体揭示了 200,000 道尔顿和 150,000 道尔顿神经丝多肽存在异质性。在免疫印迹中，单克隆抗体 iC8 与 CNS 中的 200,000 道尔顿和 150,000 道尔顿成分发生反应，但仅与 150,000 道尔顿成分发生反应。坐骨神经制剂中的多肽。此外，150,000道尔顿的多肽在坐骨神经中以单条带的形式出现，而在中枢神经系统中，双联体被iC8标记。相比之下，第二种单克隆抗体 (3H5) 与中枢和周围神经系统制剂中的 200,000 道尔顿肽和单个 150,000 道尔顿成分发生反应。 iC8 揭示的差异可能不是由于磷酸化造成的，因为免疫印迹中抗体结合的模式并未因碱性磷酸酶预处理而改变。研究结果表明神经丝多肽的不同亚型存在于神经系统中。
Abstract: A monoclonal antibody obtained from mice immunized with a crude neurofilament preparation from newborn rat brain revealed the existence of heterogeneity of the 200,000‐ and 150,000‐dalton neurofilament polypeptides. On immunoblot the monoclonal antibody iC8 reacted with both the 200,000‐ and 150,000‐dalton components in the CNS, but only with the 150,000‐dalton. polypeptide in sciatic nerve preparations. In addition, the 150,000‐dalton polypeptide appeared as a single band in the sciatic nerve, whereas in the CNS a doublet was labeled by iC8. In contrast a second monoclonal antibody (3H5) reacted with the 200,000‐dalton peptide and a single 150,000‐dalton component in both the central and peripheral nervous system preparations. The differences revealed by iC8 were probably not due to phosphorylation, as the pattern of antibody binding in immunoblots was not changed by pretreatment with alkaline phosphatase. The findings suggest that different isoforms of neurofilament polypeptides are present in the nervous system.
单克隆抗体可识别单个神经丝三联蛋白。
DOI：
10.1073/pnas.79.19.6089
发表时间：
1982
影响因子：
11.1
作者：
Lee,V;Wu,HL;Schlaepfer,WW
通讯作者：
Schlaepfer,WW
哺乳动物大脑中神经丝的分离和表征。
影响因子：
2.9
作者：
Runge,MS;Schlaepfer,WW;WilliamsJr,RC
通讯作者：
WilliamsJr,RC
影响因子：
2.9
作者：
Tokutake,S;Hutchison,SB;Pachter,JS;Liem,RK
通讯作者：
Liem,RK