Electrophoretic separations in poly(dimethylsiloxane) microchips using mixtures of ionic, nonionic and zwitterionic surfactants.
Electrophoretic separations in poly(dimethylsiloxane) microchips using mixtures of ionic, nonionic and zwitterionic surfactants.
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DOI：
10.1002/elps.201200255
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发表时间：
2012-09
期刊：
影响因子：
2.9
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
--
作者：
Guan, Qian;Noblitt, Scott D.;Henry, Charles S.
本文报道了在聚二甲基硅氧烷（PDMS）微芯片电泳中，表面活性剂混合物对电渗流和分离选择性的影响，并将电容耦合非接触电导检测（C4D）引入PDMS微芯片上的电渗流测量。首先，测量了两种非离子表面活性剂（Tween 20和Triton X-100）、混合离子/非离子表面活性剂体系（SDS/Tween 20和SDS/Triton X-100），最后首次测量了混合两性离子/非离子表面活性剂体系（TDAPS/Tween 20和TDAPS/Triton X-100）。随着表面活性剂浓度的增加，非离子表面活性剂的溶解度降低。在非离子表面活性剂中加入SDS或TDAPS可提高乳化效率。在建立了该模型后，对模型化合物的分离进行了探索，以显示其对分离的影响。与单一表面活性剂系统相比，含有吐温20和TDAPS的混合表面活性剂系统实现了具有更大峰高的类似分析物分辨率。最后，通过用80 mM K+刺激从PC 12细胞释放的儿茶酚胺的检测被执行，以证明混合表面活性剂系统提供复杂样品中的生物化合物的分辨率的有用性。
The use of surfactant mixtures to affect both electroosmotic flow (EOF) and separation selectivity in electrophoresis with poly(dimethylsiloxane) (PDMS) substrates is reported, and capacitively coupled contactless conductivity detection (C4D) is introduced for EOF measurement on PDMS microchips. First, the EOF was measured for two nonionic surfactants (Tween 20 and Triton X-100), mixed ionic/nonionic surfactant systems (SDS/Tween 20 and SDS/Triton X-100), and finally for the first time, mixed zwitterionic/nonionic surfactant systems (TDAPS/Tween 20 and TDAPS/Triton X-100). EOF for the nonionic surfactants decreased with increasing surfactant concentration. The addition of SDS or TDAPS to a nonionic surfactant increased EOF. After establishing the EOF behavior, the separation of model catecholamines was explored to show the impact on separations. Similar analyte resolution with greater peak heights was achieved with mixed surfactant systems containing Tween 20 and TDAPS relative to the single surfactant system. Finally, the detection of catecholamine release from PC12 cells by stimulation with 80 mM K+ was performed to demonstrate the usefulness of mixed surfactant systems to provide resolution of biological compounds in complex samples.
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影响因子：
2.9
作者：
Guan, Qian;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
2.9
作者：
Guan, Qian;Noblitt, Scott D.;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
4.2
作者：
Ding, Yongsheng;Mora, Maria F.;Garcia, Carlos D.
通讯作者：
Garcia, Carlos D.
影响因子：
2.9
作者：
Hoeman, Kurt W.;Culbertson, Christopher T.
通讯作者：
Culbertson, Christopher T.
影响因子：
7.4
作者：
LEE, CS;MCMANIGILL, D;PATEL, B
通讯作者：
PATEL, B