Electrophoretic separations in poly(dimethylsiloxane) microchips using a mixture of ionic and zwitterionic surfactants.
Electrophoretic separations in poly(dimethylsiloxane) microchips using a mixture of ionic and zwitterionic surfactants.
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DOI：
10.1002/elps.201100259
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发表时间：
2012-01
期刊：
影响因子：
2.9
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
--
作者：
Guan, Qian;Noblitt, Scott D.;Henry, Charles S.
关键词：
报道了在聚二甲基硅氧烷（PDMS）微芯片中使用离子型和两性离子型表面活性剂的混合物。研究了单一阴离子表面活性剂（十二烷基硫酸钠，SDS）、单一两性离子表面活性剂（N-十四烷基铵-N，N-二甲基-3-铵基-1-丙磺酸盐，TDAPS）以及SDS/TDAPS混合表面活性剂体系中表面活性剂浓度对胶束增溶的影响。SDS增加了先前报告的β-淀粉样蛋白，而TDAPS显示β-淀粉样蛋白最初增加，随后在较高浓度下减少。当TDAPS被添加到含有SDS的溶液中时，Δ以浓度依赖性方式降低。所有三种表面活性剂体系的pH值遵循预期的pH值趋势，在较高的pH值下增加pH值。混合表面活性剂体系允许在一定范围的pH值和浓度条件下调节pH值。在建立了吸附行为后，测定了吸附/解吸动力学，结果表明TDAPS的吸附/解吸速率比SDS慢。最后，研究了混合表面活性剂体系对缓冲液中模型儿茶酚胺和红细胞裂解液中还原型谷胱甘肽（GSH）的分离和电化学检测。与单一表面活性剂体系相比，混合表面活性剂体系提供更短的分析时间和/或提高的分辨率。
The use of mixtures of ionic and zwitterionic surfactants in poly(dimethylsiloxane) (PDMS) microchips is reported. The effect of surfactant concentration on EOF was studied for a single anionic surfactant (sodium dodecyl sulfate, SDS), a single zwitterionic surfactant (N-tetradecylammonium-N,N-dimethyl-3-ammonio-1-propanesulfonate, TDAPS), and a mixed SDS/TDAPS surfactant system. SDS increased the EOF as reported previously while TDAPS showed an initial increase in EOF followed by a reduction at higher concentrations. When TDAPS was added to a solution containing SDS, the EOF decreased in a concentration dependent manner. The EOF for all three surfactant systems followed expected pH trends, with increasing EOF at higher pH. The mixed surfactant system allowed tuning of the EOF across a range of pH and concentration conditions. After establishing the EOF behavior, the adsorption/desorption kinetics were measured and showed a slower adsorption/desorption rate for TDAPS than SDS. Finally, the separation and electrochemical detection of model catecholamines in buffer and reduced glutathione (GSH) in red blood cell lysate using the mixed surfactant system were explored. The mixed surfactant system provided shorter analysis times and/or improved resolution when compared to the single surfactant systems.
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影响因子：
7.4
作者：
García, CD;Henry, CS
通讯作者：
Henry, CS
影响因子：
2.9
作者：
Guan, Qian;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
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作者：
Hu, SW;Ren, XQ;Allbritton, N
通讯作者：
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影响因子：
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作者：
Garcia, CD;Henry, CS
通讯作者：
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作者：
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通讯作者：
GRIFFITH, OW