Improving MCE with electrochemical detection using a bubble cell and sample stacking techniques.
Improving MCE with electrochemical detection using a bubble cell and sample stacking techniques.
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DOI：
10.1002/elps.200900316
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发表时间：
2009-10
期刊：
影响因子：
2.9
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
--
作者：
Guan, Qian;Henry, Charles S.
关键词：
本文介绍了提高电化学检测MCE的灵敏度和检测限的两项努力。一是在检测区实施毛细管膨胀（气泡池），以增加暴露的工作电极表面积。气泡池宽度为分离通道宽度（50 μm）的1 ~ 10倍，研究电极表面积对检测灵敏度、LOD和分离效率的影响。随着泡细胞宽度的增加，检测灵敏度提高，检出限降低，在5倍泡细胞中检测到的多巴胺和儿茶酚的lod分别为25 nM和50 nM。同时，荧光成像结果显示，在4×和5×泡池中，分离效率分别损失约8%和约12%。另一种降低LOD的方法是使用场放大样品进样（FASI）进行门控进样，使用场放大样品堆积（FASS）进行流体动力进样。研究了安培检测和脉冲安培检测两种方法的叠加效应。FASI法和FASS法对4×泡细胞多巴胺的LODs分别为8 nM和20 nM。然而，无论使用哪一种堆叠技术，阴离子分析物的lod都没有得到改善。
Two efforts to improve the sensitivity and limits of detection for MCE with electrochemical detection are presented here. One is the implementation of a capillary expansion (bubble cell) at the detection zone to increase the exposed working electrode surface area. Bubble cell widths were varied from 1× to 10× the separation channel width (50 μm) to investigate the effects of electrode surface area on detection sensitivity, LOD, and separation efficiency. Improved detection sensitivity and decreased detection limits were obtained with increased bubble cell width, and LODs of dopamine and catechol detected in a 5× bubble cell were 25 nM and 50 nM, respectively. Meanwhile, fluorescent imaging results demonstrated ~8% and ~12% loss in separation efficiency in 4× and 5× bubble cell, respectively. Another effort for reducing the LOD involves using field amplified sample injection (FASI) for gated injection and field amplified sample stacking (FASS) for hydrodynamic injection. Stacking effects are shown for both methods using amperometric detection and pulsed amperometric detection (PAD). The LODs of dopamine in a 4× bubble cell were 8 nM and 20 nM using FASI and FASS, respectively. However, improved LODs were not obtained for anionic analytes using either stacking technique.
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影响因子：
6.2
作者：
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