A novel intravital method to evaluate cerebral vasospasm in rat models of subarachnoid hemorrhage: a study with synchrotron radiation angiography.
A novel intravital method to evaluate cerebral vasospasm in rat models of subarachnoid hemorrhage: a study with synchrotron radiation angiography.
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一种评估蛛网膜下腔出血大鼠模型脑血管痉挛的新活体方法：同步辐射血管造影研究
DOI：
10.1371/journal.pone.0033366
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ling F
中科院分区：
文献类型：
--
作者：
Cai J;Sun Y;Yuan F;Chen L;He C;Bao Y;Chen Z;Lou M;Xia W;Yang GY;Ling F
在实验性小动物模型中，精确评估蛛网膜下腔出血引起的脑血管痉挛仍然是一个关键但尚未解决的问题。在本研究中，我们使用同步辐射血管造影技术研究了两种大鼠蛛网膜下腔出血模型中前循环动脉的血管痉挛。采用同步辐射血管造影、激光多普勒血流仪-脑血流测定、[125I]N-异丙基对碘苯丙胺脑血流测定及终末检查等方法评价小脑延髓池和视交叉前池注血制作的2种蛛网膜下腔出血模型前循环动脉的变化。使用同步辐射血管造影技术，我们检测到蛛网膜下腔出血大鼠与对照组相比有脑血管痉挛（p<0.05）。我们还发现了两个有趣的发现：1）大脑中动脉和大脑前动脉在蛛网膜下腔出血后第3天收缩最多； 2)视交叉前池注射组大脑前动脉直径小于小脑延脑池注射组(p<0.05)，但大脑中动脉直径不小于小脑池注射组。我们的结论是，同步辐射血管造影提供了一种新技术，可以直接评估小动物实验性蛛网膜下腔出血模型中的脑血管痉挛。这两种注射模型的血管痉挛过程相似；然而，交叉前池注射制作的模型更适合前循环血管痉挛的研究。
Precise in vivo evaluation of cerebral vasospasm caused by subarachnoid hemorrhage has remained a critical but unsolved issue in experimental small animal models. In this study, we used synchrotron radiation angiography to study the vasospasm of anterior circulation arteries in two subarachnoid hemorrhage models in rats. Synchrotron radiation angiography, laser Doppler flowmetry-cerebral blood flow measurement, [125I]N-isopropyl-p-iodoamphetamine cerebral blood flow measurement and terminal examinations were applied to evaluate the changes of anterior circulation arteries in two subarachnoid hemorrhage models made by blood injection into cisterna magna and prechiasmatic cistern. Using synchrotron radiation angiography technique, we detected cerebral vasospasm in subarachnoid hemorrhage rats compared to the controls (p<0.05). We also identified two interesting findings: 1) both middle cerebral artery and anterior cerebral artery shrunk the most at day 3 after subarachnoid hemorrhage; 2) the diameter of anterior cerebral artery in the prechiasmatic cistern injection group was smaller than that in the cisterna magna injection group (p<0.05), but not for middle cerebral artery. We concluded that synchrotron radiation angiography provided a novel technique, which could directly evaluate cerebral vasospasm in small animal experimental subarachnoid hemorrhage models. The courses of vasospasm in these two injection models are similar; however, the model produced by prechiasmatic cistern injection is more suitable for study of anterior circulation vasospasm.
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影响因子：
3.3
作者：
Cetas, J. S.;Lee, D. R.;Alkayed, N. J.;Wang, R.;Iliff, J. J.;Heinricher, M. M.
通讯作者：
Heinricher, M. M.
影响因子：
8.3
作者：
GARCIA, JH;WAGNER, S;HU, XJ
通讯作者：
HU, XJ
影响因子：
3.5
作者：
Elleaume, H;Fiedler, S;Le Bas, JF
通讯作者：
Le Bas, JF
DOI：
10.1152/ajpheart.00616.2002
发表时间：
2002-12-01
影响因子：
4.8
作者：
Gules, I;Satoh, M;Zhang, JH
通讯作者：
Zhang, JH
影响因子：
2.4
作者：
Aladag, MA;Turkoz, Y;Gul, M
通讯作者：
Gul, M