Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus.
Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus.
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金黄色葡萄球菌 saeS 中单核苷酸变化对 FtsH 调控网络的重新布线
DOI：
10.1038/s41598-017-08774-5
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发表时间：
2017-08-16
影响因子：
4.6
通讯作者：
Bae T
中科院分区：
文献类型：
--
作者：
Liu Q;Hu M;Yeo WS;He L;Li T;Zhu Y;Meng H;Wang Y;Lee H;Liu X;Li M;Bae T
在革兰氏阳性金黄色葡萄球菌中，膜结合的三磷酸腺苷依赖的金属蛋白酶FtsH在抵抗各种应激源方面起着关键作用。然而，FtsH功能的分子机制尚不清楚。在这里，我们鉴定了金黄色葡萄球菌的核心FtsH靶蛋白。在Newman和USA300菌株中，33个蛋白的丰度发生了变化，其中11个被鉴定为核心FtsH底物蛋白候选。在纽曼菌株和其他一些金黄色葡萄球菌菌株中，传感器组氨酸激酶SAES具有L18P(SAEs中的T53C)替换，该替换将蛋白质转化为FtsH底物。由于FtsH突变体中SAES L18P的增加，SAE调节蛋白EAP也大量增加，导致了纽曼特有的细胞聚集表型。然而，无论菌株背景如何，FtsH突变体在小鼠感染模型中显示出较低的毒力和存活率。我们的研究说明了细菌调控网络的弹性，它可以通过一个单一的替换突变来重新连接。
In the Gram-positive pathogen Staphylococcus aureus, the membrane-bound ATP-dependent metalloprotease FtsH plays a critical role in resistance to various stressors. However, the molecular mechanism of the FtsH functions is not known. Here, we identified core FtsH target proteins in S. aureus. In the strains Newman and USA300, the abundance of 33 proteins were altered in both strains, of which 11 were identified as core FtsH substrate protein candidates. In the strain Newman and some other S. aureus strains, the sensor histidine kinase SaeS has an L18P (T53C in saeS) substitution, which transformed the protein into an FtsH substrate. Due to the increase of SaeS L18P in the ftsH mutant, Eap, a sae-regulon protein, was also increased in abundance, causing the Newman-specific cell-aggregation phenotype. Regardless of the strain background, however, the ftsH mutants showed lower virulence and survival in a murine infection model. Our study illustrates the elasticity of the bacterial regulatory network, which can be rewired by a single substitution mutation.
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影响因子：
16
作者：
Herman, C;Prakash, S;Gross, CA
通讯作者：
Gross, CA
影响因子：
2.8
作者：
Harraghy, N;Kormanec, J;Herrmann, M
通讯作者：
Herrmann, M
DOI：
10.1099/00221287-6-1-2-95
发表时间：
1952-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
DUTHIE, ES;LORENZ, LL
通讯作者：
LORENZ, LL
影响因子：
11.4
作者：
Alix, Eric;Blanc-Potard, Anne-Beatrice
通讯作者：
Blanc-Potard, Anne-Beatrice
影响因子：
3.6
作者：
Jeong DW;Cho H;Jones MB;Shatzkes K;Sun F;Ji Q;Liu Q;Peterson SN;He C;Bae T
通讯作者：
Bae T