Stem Cells Grown in Osteogenic Medium on PLGA, PLGA/HA, and Titanium Scaffolds for Surgical Applications.
Stem Cells Grown in Osteogenic Medium on PLGA, PLGA/HA, and Titanium Scaffolds for Surgical Applications.
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用于手术应用的PLGA，PLGA/HA和钛支架上的成骨培养基中生长的干细胞。
DOI：
10.1155/2010/831031
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发表时间：
2010
影响因子：
3.8
通讯作者：
Benazzo F
中科院分区：
文献类型：
--
作者：
Asti A;Gastaldi G;Dorati R;Saino E;Conti B;Visai L;Benazzo F
多能脂肪组织来源干细胞(HASCs)可以分化为多种中胚层细胞类型，如成骨细胞、软骨母细胞和成肌细胞。我们从骨科手术期间的皮下脂肪组织中分离出hASCs，并在三种不同的合成支架聚乳酸-乙交酯(PLGA)、聚乳酸-乙交酯/羟基磷灰石(PLGA/HA)和小梁钛支架(Ti6Al4V)上进行了28天的成骨诱导分化。孔径大小会影响某些指标，如细胞附着、渗透和血管形成。本研究的目的是研究PLGA和PLGA/HA支架的孔隙率在75%~84%之间的性能，与钛支架相比，种植hASCs的PLGA和PLGA/HA支架的孔尺寸较小，以建立一种可用于骨缺损和骨折治疗的模型。通过ELISA定量细胞外基质蛋白表达、von Kossa染色、X射线显微分析和扫描电子显微镜评价成骨能力。钛支架上的蛋白质基质相对于PLGA和PLGA/HA的含量较高，这一结论表明，不仅材料类型而且结构对细胞增殖有显着影响。
Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal cell types such as osteoblasts, chondroblasts, and myoblasts. We isolated hASCs from subcutaneous adipose tissue during orthopaedic surgery and induced the osteogenic differentiation for 28 days on three different synthetic scaffolds such as polylactide-co-glycolide (PLGA), polylactide-co-glycolide/hydroxyapatite (PLGA/HA), and trabecular titanium scaffolds (Ti6Al4V). Pore size can influence certain criteria such as cell attachment, infiltration, and vascularization. The aim of this study was to investigate the performance of PLGA and PLGA/HA scaffolds with a higher porosity, ranging between 75% and 84%, with respect to Ti scaffolds but with smaller pore size, seeded with hASCs to develop a model that could be used in the treatment of bone defects and fractures. Osteogenesis was assessed by ELISA quantitation of extracellular matrix protein expression, von Kossa staining, X-ray microanalysis, and scanning electron microscopy. The higher amount of protein matrix on the Ti scaffold with respect to PLGA and PLGA/HA leads to the conclusion that not only the type of material but the structure significantly affects cell proliferation.
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影响因子：
4.9
作者：
Frosch, KH;Barvencik, F;Stürmer, KM
通讯作者：
Stürmer, KM
DOI：
10.1016/j.injury.2007.02.008
发表时间：
2007-03-01
影响因子：
2.5
作者：
Carson, Joshua S.;Bostrom, Mathias P. G.
通讯作者：
Bostrom, Mathias P. G.
影响因子：
4.9
作者：
Li, JP;Li, SH;de Groot, K
通讯作者：
de Groot, K
DOI：
10.1016/j.jmbbm.2010.02.001
发表时间：
2010-07-01
影响因子：
3.9
作者：
Marin, E.;Fusi, S.;Fearizzi, L.
通讯作者：
Fearizzi, L.
影响因子：
5.6
作者：
OWEN, TA;ARONOW, M;STEIN, GS
通讯作者：
STEIN, GS