Liquid-cell transmission electron microscopy for imaging of thermosensitive recombinant polymers.
Liquid-cell transmission electron microscopy for imaging of thermosensitive recombinant polymers.
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DOI：
10.1016/j.jconrel.2022.02.019
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发表时间：
2022-04
影响因子：
10.8
通讯作者：
Ghandehari, Hamidreza
中科院分区：
文献类型：
--
作者：
Isaacson, Kyle J.;Van Devener, Brian R.;Steinhauff, Douglas B.;Jensen, M. Martin;Cappello, Joseph;Ghandehari, Hamidreza
关键词：
在控制释放应用中使用的各种聚合物表现出基于溶液的热响应。不幸的是，很少有表征和成像技术允许在单个聚合物的热触发相变过程中进行分辨率分析。在这里，我们展示了使用温度斜坡液池透射电子显微镜（LCTEM）来实时评估弹性蛋白样多肽（ELPs）及其自组装纳米结构在包含其固有低临界溶液温度（LCSTs）的温度范围内的溶液和界面行为。根据溶解度来区分单个聚合物和超分子组合。结果表明，重组聚合物可以从缓冲盐水溶液中吸附到氮化硅芯片窗口，并以温度依赖的方式解吸。丝绸-弹性蛋白样蛋白嵌段共聚物（selp）由丝绸和弹性蛋白的重复肽基序组成，其热行为与elp不同。虽然两种聚合物都显示成簇，但只有selp在加热时形成坚固的淀粉样纤维。
Various polymers used in controlled release applications exhibit solution-based thermal responses. Unfortunately, very few characterization and imaging techniques permit resolution of individual polymers during their thermally-triggered phase transitions. Here, we demonstrate the use of temperature-ramp liquid-cell transmission electron microscopy (LCTEM) for real-time evaluation of the solution and interfacial behavior of elastinlike polypeptides (ELPs) and their self-assembled nanostructures over a temperature range incorporating their intrinsic lower critical solution temperatures (LCSTs). Individual polymers and supramolecular assemblies were discriminated dependent upon solubility states. The recombinant polymers were shown to adsorb to the silicon-nitride chip window from the buffered saline solution and desorb in a temperature-dependent manner. Silk-elastinlike protein block copolymers (SELPs) (composed of repeat peptide motifs of silk and elastin) differed from ELPs in thermal behavior. While both polymers were shown to cluster, only SELPs formed robust amyloid-like fibers upon heating.
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影响因子：
16.6
作者：
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影响因子：
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作者：
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通讯作者：
Ghandehari, Hamidreza
影响因子：
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作者：
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通讯作者：
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