Distinct mechanisms regulate hemocyte chemotaxis during development and wound healing in Drosophila melanogaster.
Distinct mechanisms regulate hemocyte chemotaxis during development and wound healing in Drosophila melanogaster.
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DOI：
10.1083/jcb.200508161
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发表时间：
2006-05-08
期刊：
影响因子：
--
通讯作者：
Jacinto A
中科院分区：
文献类型：
--
作者：
Wood W;Faria C;Jacinto A
黑腹果蝇血细胞是高度能动的巨噬细胞样细胞，其经历定型的迁移模式以通过晚期胚胎发生填充整个胚胎。我们证明，在胚胎腹侧中线的血细胞的迁移模式是精心策划的PDGF/VEGF配体Pvf 2和-3的趋化信号，这些定向迁移发生独立的磷脂酰肌醇3-激酶（PI 3 K）信号。相比之下，使用激光消融和一种新的创伤检测，允许局部治疗与抑制性药物，我们表明，PI 3 K是必不可少的血细胞趋化性对伤口和Pvf信号和PDGF/VEGF受体表达是不需要这种快速的趋化反应。我们的研究结果表明，至少有两个独立的机制在D。黑腹动物胚胎中的PI 3 K来指导血细胞迁移，并表明尽管PI 3 K对于血细胞感知来自伤口的趋化梯度是至关重要的，但不需要感知在胚胎发生期间协调它们沿着腹侧中线的发育迁移的生长因子信号。
Drosophila melanogaster hemocytes are highly motile macrophage-like cells that undergo a stereotypic pattern of migration to populate the whole embryo by late embryogenesis. We demonstrate that the migratory patterns of hemocytes at the embryonic ventral midline are orchestrated by chemotactic signals from the PDGF/VEGF ligands Pvf2 and -3 and that these directed migrations occur independently of phosphoinositide 3-kinase (PI3K) signaling. In contrast, using both laser ablation and a novel wounding assay that allows localized treatment with inhibitory drugs, we show that PI3K is essential for hemocyte chemotaxis toward wounds and that Pvf signals and PDGF/VEGF receptor expression are not required for this rapid chemotactic response. Our results demonstrate that at least two separate mechanisms operate in D. melanogaster embryos to direct hemocyte migration and show that although PI3K is crucial for hemocytes to sense a chemotactic gradient from a wound, it is not required to sense the growth factor signals that coordinate their developmental migrations along the ventral midline during embryogenesis.
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