Diversity and classification of cyclic-oligonucleotide-based anti-phage signalling systems.
Diversity and classification of cyclic-oligonucleotide-based anti-phage signalling systems.
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DOI：
10.1038/s41564-020-0777-y
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发表时间：
2020-12
影响因子：
28.3
通讯作者：
Sorek R
中科院分区：
文献类型：
--
作者：
Millman A;Melamed S;Amitai G;Sorek R
基于环状寡核苷酸的抗噬菌体信号系统 (CBASS) 是一个抗噬菌体防御系统家族，与动物 cGAS-STING 先天免疫途径有共同的祖先。 CBASS系统由寡核苷酸环化酶和效应器组成，寡核苷酸环化酶响应噬菌体感染而产生信号环状寡核苷酸，效应器由环状寡核苷酸激活并促进细胞死亡。细胞死亡发生在噬菌体复制完成之前，从而防止噬菌体扩散到附近的细胞。在这里，我们分析了 38,000 个细菌和古细菌基因组，并鉴定了 5,000 多个 CBASS 系统，这些系统具有多种结构，包括多个信号分子、效应子和辅助基因。我们提出了 CBASS 的分类系统，该系统根据操纵子组织、信号分子和效应器功能对系统进行分组。确定了四种主要的 CBASS 类型，它们共享至少六种通过膜损伤、DNA 降解或其他方式促进细胞死亡的效应子亚型。我们观察到 CBASS 系统广泛增益和丢失的证据，以及系统之间效应基因的改组。我们的分类和命名方案预计将指导正在发展的 CBASS 领域的未来研究。
Cyclic oligonucleotide based anti-phage signaling systems (CBASS) are a family of anti-phage defense systems that share ancestry with the animal cGAS-STING innate immune pathway. CBASS systems are composed of an oligonucleotide cyclase, which generates signaling cyclic oligonucleotides in response to phage infection, and an effector that is activated by the cyclic oligonucleotides and promotes cell death. Cell death occurs prior to the completion of phage replication, thus preventing the spread of phages to nearby cells. Here we analysed 38,000 bacterial and archaeal genomes and identified over 5,000 CBASS systems, which have diverse architectures with multiple signaling molecules, effectors and ancillary genes. We propose a classification system for CBASS that groups systems according to their operon organization, signaling molecules and effector function. Four major CBASS types were identified, sharing at least six effector subtypes that promote cell death by membrane impairment, DNA degradation or other means. We observed evidence of extensive gain and loss of CBASS systems, as well as shuffling of effector genes between systems. Our classification and nomenclature scheme is expected to guide future research in the developing CBASS field.
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影响因子：
10.7
作者：
Price MN;Dehal PS;Arkin AP
通讯作者：
Arkin AP
影响因子：
14.9
作者：
Chen IA;Chu K;Palaniappan K;Pillay M;Ratner A;Huang J;Huntemann M;Varghese N;White JR;Seshadri R;Smirnova T;Kirton E;Jungbluth SP;Woyke T;Eloe-Fadrosh EA;Ivanova NN;Kyrpides NC
通讯作者：
Kyrpides NC
影响因子：
28.3
作者：
Ofir G;Melamed S;Sberro H;Mukamel Z;Silverman S;Yaakov G;Doron S;Sorek R
通讯作者：
Sorek R
影响因子：
16
作者：
Lau, Rebecca K.;Ye, Qiaozhen;Corbett, Kevin D.
通讯作者：
Corbett, Kevin D.
影响因子：
2.9
作者：
McCarty, Reid M.;Somogyi, Arpad;Lin, Guangxin;Jacobsen, Neil E.;Bandarian, Vahe
通讯作者：
Bandarian, Vahe