Cell-cell signaling in co-cultures of macrophages and fibroblasts.
Cell-cell signaling in co-cultures of macrophages and fibroblasts.
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DOI：
10.1016/j.biomaterials.2010.07.101
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发表时间：
2010-12
期刊：
影响因子：
14
通讯作者：
Grainger, David W.
中科院分区：
文献类型：
--
作者：
Holt, Dolly J.;Chamberlain, Lisa M.;Grainger, David W.
异物反应（FBR）包括一种普遍的、普遍存在的基于宿主组织的对植入材料的反应。体外细胞模型经常被用于研究涉及细胞对材料信号反应的FBR机制。然而，这些模型往往只研究一种细胞类型，只识别有限的信号，不能准确地代表体内炎症信号的复杂性。为了解决这个问题，采用了一种细胞共培养系统，其中包括两种主要的FBR效应细胞，巨噬细胞和成纤维细胞。监测这些细胞类型之间的细胞-细胞信号系统，包括长期1)在条件培养基中从另一种细胞类型培养一种细胞类型，2)非接触细胞共培养（旁分泌信号），以及3)原代和继代细胞的接触共培养（旁分泌信号）。在第1、2、3、7、14和21天收集细胞培养基和细胞图像，评估可溶性蛋白分泌、细胞行为和形态的变化。原代和次级衍生细胞在每种信号传导情况下和彼此之间都有独特的反应。一般来说，与单一培养和所有继代细胞培养相比，原代细胞共培养对fbr样反应的体外保真度更高。
The foreign body response (FBR) comprises a general, ubiquitous host tissue-based reaction to implanted materials. In vitro cell-based models are frequently employed to study FBR mechanisms involving cell signaling responses to materials. However, these models often study only one cell type, identify only limited signals, and cannot accurately represent the complexity of in vivo inflammatory signaling. To address this issue, a cell co-culture system involving two primary effector cells of the FBR, macrophages and fibroblasts, was employed. Cell-cell signaling systems were monitored between these cell types, including long-term 1) culture of one cell type in conditioned media from the other cell type, 2) non-contacting cell co-cultures (paracrine signaling), and 3) contact co-cultures (juxtacrine signaling) of primary- and secondary-derived cells. Cell culture media and cell images were collected on Days 1, 2, 3, 7, 14, and 21 and changes in soluble protein secretion, cellular behavior, and morphology were assessed. Primary- and secondary- derived cells responded uniquely during each signaling scenario and to one another. In general higher in vitro fidelity to FBR-like responses was found in primary cell co-cultures compared to their mono-cultures and all secondary cell cultures.
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影响因子：
3.7
作者：
HAYFLICK, L;MOORHEAD, PS
通讯作者：
MOORHEAD, PS
DOI：
10.1006/bbrc.1998.8261
发表时间：
1998-03-17
影响因子：
3.1
作者：
Isumi, Y;Minamino, N;Matsuo, H
通讯作者：
Matsuo, H
DOI：
10.1163/156856206778530731
发表时间：
2006-01-01
影响因子：
3.6
作者：
Godek, M. L.;Sampson, J. A.;Grainger, D. W.
通讯作者：
Grainger, D. W.
影响因子：
5.4
作者：
Helming, Laura;Gordon, Siamon
通讯作者：
Gordon, Siamon
DOI：
10.1163/156856206777346340
发表时间：
2006-01-01
影响因子：
3.6
作者：
Gretzer, Christina;Emanuelsson, Lena;Thomsen, Peter
通讯作者：
Thomsen, Peter