GaMYB85, an R2R3 MYB gene, in transgenic Arabidopsis plays an important role in drought tolerance.
GaMYB85, an R2R3 MYB gene, in transgenic Arabidopsis plays an important role in drought tolerance.
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GamyB85是转基因拟南芥中的R2R3 MYB基因，在干旱耐受性中起着重要作用。
DOI：
10.1186/s12870-017-1078-3
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发表时间：
2017-08-22
影响因子：
5.3
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Butt HI;Yang Z;Gong Q;Chen E;Wang X;Zhao G;Ge X;Zhang X;Li F
MYB转录因子是高等植物中最大的转录因子家族之一，参与多种生物学、功能和结构过程。以前，很少有功能验证研究R2 R3 MYB已在棉花响应非生物胁迫。在目前的研究中，GaMYB 85，棉花R2 R3 MYB TF，异位表达拟南芥（Col-0）和功能的特点是在转基因植物中过表达。GaMYB 85的计算机分析显示存在具有保守的R2 R3 MYB不完全重复的SANT结构域。GaMYB 85蛋白质具有257个氨基酸的序列，分子量为24.91kD，等电点为5.58。过表达GaMYB 85的拟南芥植株在甘露醇和盐胁迫下表现出更高的种子萌发率，并且在缺水时比野生型植株表现出更高的耐旱效率。这些植物有显着较高的游离脯氨酸和叶绿素水平，随后较低的水分损失率和较高的相对含水量。GaMYB 85转基因植株的萌发对脱落酸（阿坝）更敏感，极容易受到ABA诱导的主根伸长抑制。此外，当受到不同浓度的阿坝处理时，异位表达GaMYB 85的转基因植物表现出气孔密度降低，气孔大小和气孔开放率低于野生型植物。GaMYB 85的异位表达导致应激相关标记基因如RD 22、ADH 1、RD 29 A、P5 CS和ABI 5的转录水平增强。 我们的研究结果表明，GaMYB 85以前未知的作用，表明它赋予良好的干旱，盐和冷冻耐受性，最有可能通过ABA诱导的途径。这些发现可能被利用来开发棉花植物的非生物胁迫耐受性。本文的在线版本（doi：10.1186/s12870-017-1078-3）包含补充材料，可供授权用户使用。
MYB transcription factors (TFs) are one of the largest families of TFs in higher plants and are involved in diverse biological, functional, and structural processes. Previously, very few functional validation studies on R2R3 MYB have been conducted in cotton in response to abiotic stresses. In the current study, GaMYB85, a cotton R2R3 MYB TF, was ectopically expressed in Arabidopsis thaliana (Col-0) and was functionally characterized by overexpression in transgenic plants. The in-silico analysis of GaMYB85 shows the presence of a SANT domain with a conserved R2R3 MYB imperfect repeat. The GaMYB85 protein has a 257-amino acid sequence, a molecular weight of 24.91 kD, and an isoelectric point of 5.58. Arabidopsis plants overexpressing GaMYB85 exhibited a higher seed germination rate in response to mannitol and salt stress, and higher drought avoidance efficiency than wild-type plants upon water deprivation. These plants had notably higher levels of free proline and chlorophyll with subsequent lower water loss rates and higher relative water content. Germination of GaMYB85 transgenics was more sensitive to abscisic acid (ABA) and extremely liable to ABA-induced inhibition of primary root elongation. Moreover, when subjected to treatment with different concentrations of ABA, transgenic plants with ectopically expressed GaMYB85 showed reduced stomatal density, with greater stomatal size and lower stomatal opening rates than those in wild-type plants. Ectopic expression of GaMYB85 led to enhanced transcript levels of stress-related marker genes such as RD22, ADH1, RD29A, P5CS, and ABI5. Our results indicate previously unknown roles of GaMYB85, showing that it confers good drought, salt, and freezing tolerance, most probably via an ABA-induced pathway. These findings can potentially be exploited to develop improved abiotic stress tolerance in cotton plants. The online version of this article (doi:10.1186/s12870-017-1078-3) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
He Q;Jones DC;Li W;Xie F;Ma J;Sun R;Wang Q;Zhu S;Zhang B
通讯作者：
Zhang B
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
6.5
作者：
Cheng, Liqin;Li, Xiaoxia;Liu, Gongshe
通讯作者：
Liu, Gongshe
DOI：
10.1073/pnas.0604882103
发表时间：
2006-08-29
影响因子：
11.1
作者：
Hu, Honghong;Dai, Mingqiu;Xiong, Lizhong
通讯作者：
Xiong, Lizhong