Evaluation of velvet antler total protein effect on bone marrow‑derived endothelial progenitor cells.
Evaluation of velvet antler total protein effect on bone marrow‑derived endothelial progenitor cells.
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鹿茸总蛋白对骨髓源性内皮祖细胞影响的评价
DOI：
10.3892/mmr.2017.7019
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发表时间：
2017-09
影响因子：
3.4
通讯作者：
Zheng X
中科院分区：
文献类型：
--
作者：
Xiao X;Li L;Xu S;Mao M;Pan R;Li Y;Wu J;Huang L;Zheng X
鹿茸，鹿茸（VA），是一种传统中药，在中国、日本、俄罗斯、新西兰和东南亚被用作食品补充剂和治疗药物。VA的再生特性引起了极大的研究兴趣，特别是关于器官移植和干细胞分化的领域。各种VA蛋白质组学研究证实，蛋白质作为VA的主要生物活性成分。本研究旨在探讨VA蛋白（VA-pro）是否影响内皮祖细胞（EPC）的活力。目前已有多种方法对VA-pro进行研究，包括冷冻干燥法、超声波法、高效液相色谱-质谱法、EPCs提取和培养等。结果表明，VA-pro促进EPCs增殖和迁移，特别是在1 mg/ml的浓度下。此外，VA-pro增加Notch 1胞内结构域和Hes 1的活化水平，以及磷酸化Akt和磷酸化雷帕霉素机制靶点的水平。因此，VA-pro可能通过调节Notch和Akt信号通路影响EPC活力。本研究揭示了VA-pro对EPCs的影响及其可能的分子机制，并提示VA的再生特性与EPCs活力的优化之间存在关联。这些发现可能有助于EPC移植研究，并有助于为未来的血管疾病提供一种新的治疗方法。
Lu Rong, velvet antler (VA), is a traditional Chinese medicine, which is used as a food supplement and therapeutic drug in China, Japan, Russia, New Zealand and Southeast Asia. The regenerative characteristics of VA have resulted in great research interest, particularly regarding the fields of organ grafting and stem cell differentiation. Various VA proteomic studies verified that proteins act as the primary bioactive components of VA. The present study aimed to investigate if VA proteins (VA-pro) influence endothelial progenitor cell (EPC) viability. Various methods have previously been used to investigate VA-pro, including freeze-drying technology, ultrasonic wave methods, high performance liquid chromatography-mass spectrometry, EPCs extraction and culture. Results demonstrated that VA-pro promoted EPCs proliferation and migration, particularly at a concentration of 1 mg/ml. Furthermore, VA-pro increased the activation level of Notch1 intracellular domain and Hes1, and the level of phosphorylated-Akt and phosphorylated-mechanistic target of rapamycin. VA-pro may therefore affect EPC viability via regulation of the Notch and Akt signaling pathways. The present study revealed the effects and potential molecular mechanism of VA-pro on EPCs, and suggested an association between VA regeneration characteristics and the optimization of EPC viability. These findings may contribute to EPC transplantation research and aid in providing a novel treatment method for vascular diseases in the future.
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影响因子：
37.8
作者：
Ii M;Takeshita K;Ibusuki K;Luedemann C;Wecker A;Eaton E;Thorne T;Asahara T;Liao JK;Losordo DW
通讯作者：
Losordo DW
影响因子：
1.6
作者：
Jeon, Byongtae;Kim, Sungjin;Moon, Sangho
通讯作者：
Moon, Sangho
影响因子：
4.2
作者：
Pita-Thomas, Wolfgang;Nieto-Sampedro, Manuel;Nieto-Diaz, Manuel
通讯作者：
Nieto-Diaz, Manuel
影响因子：
39.3
作者：
Rizzo, Paola;Miele, Lucio;Ferrari, Roberto
通讯作者：
Ferrari, Roberto
影响因子：
1.5
作者：
Sugihara, Shinobu;Yamamoto, Yasutaka;Hisatome, Ichiro
通讯作者：
Hisatome, Ichiro