High-pressure open-channel on-chip electroosmotic pump for nanoflow high performance liquid chromatography.
High-pressure open-channel on-chip electroosmotic pump for nanoflow high performance liquid chromatography.
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DOI：
10.1021/ac4040345
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发表时间：
2014-02-18
影响因子：
7.4
通讯作者：
Liu, Shaorong
中科院分区：
文献类型：
--
作者：
Wang, Wei;Gu, Congying;Lynch, Kyle B.;Lu, Joann J.;Zhang, Zhengyu;Pu, Qiaosheng;Liu, Shaorong
在这里，我们构建了一个开放通道片上电渗泵，能够产生高达 ∼170 bar 的压力和高达 ∼500 nL/min 的流速，足以进行高效液相色谱 (HPLC) 分离。该泵的一大特点是可以将多个基本泵组串联起来，以增强泵的抽气功率；输出压力与连接的泵单元数量成正比。这种添加剂性质非常出色且有用，没有其他泵可以以这种方式工作。我们论证了使用该泵进行纳流 HPLC 分离的可行性；使用牛血清白蛋白（BSA）、转铁蛋白因子（TF）和人免疫球蛋白（IgG）的胰蛋白酶消化物作为示例性样品。我们还将电渗 (EO) 驱动的 HPLC 与 Agilent 1200 HPLC 的性能进行了比较；获得了可比的效率、分辨率和峰值容量。由于该泵基于电渗，因此没有移动部件。通用材料和工艺还允许该泵与其他微加工功能部件集成。这种高压片上泵的开发将对片上实验室设备的进步产生深远的影响。
Here, we construct an open-channel on-chip electroosmotic pump capable of generating pressures up to ∼170 bar and flow rates up to ∼500 nL/min, adequate for high performance liquid chromatographic (HPLC) separations. A great feature of this pump is that a number of its basic pump units can be connected in series to enhance its pumping power; the output pressure is directly proportional to the number of pump units connected. This additive nature is excellent and useful, and no other pumps can work in this fashion. We demonstrate the feasibility of using this pump to perform nanoflow HPLC separations; tryptic digests of bovine serum albumin (BSA), transferrin factor (TF), and human immunoglobulins (IgG) are utilized as exemplary samples. We also compare the performance of our electroosmotic (EO)-driven HPLC with Agilent 1200 HPLC; comparable efficiencies, resolutions, and peak capacities are obtained. Since the pump is based on electroosmosis, it has no moving parts. The common material and process also allow this pump to be integrated with other microfabricated functional components. Development of this high-pressure on-chip pump will have a profound impact on the advancement of lab-on-a-chip devices.
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影响因子：
7.4
作者：
Reichmuth, DS;Shepodd, TJ;Kirby, BJ
通讯作者：
Kirby, BJ
影响因子：
8.4
作者：
Grover, WH;Skelley, AM;Mathies, RA
通讯作者：
Mathies, RA
影响因子：
5.5
作者：
Koetse, M;Laschewsky, A;Wischerhoff, E
通讯作者：
Wischerhoff, E
影响因子：
7.4
作者：
Gu, Congying;Jia, Zhijian;Liu, Shaorong
通讯作者：
Liu, Shaorong
影响因子：
2
作者：
Nguyen, NT;Huang, XY;Chuan, TK
通讯作者：
Chuan, TK