Histone H1 subtypes differentially modulate chromatin condensation without preventing ATP-dependent remodeling by SWI/SNF or NURF.
Histone H1 subtypes differentially modulate chromatin condensation without preventing ATP-dependent remodeling by SWI/SNF or NURF.
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DOI：
10.1371/journal.pone.0007243
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发表时间：
2009-10-01
期刊：
影响因子：
3.7
通讯作者：
Beato M
中科院分区：
文献类型：
--
作者：
Clausell J;Happel N;Hale TK;Doenecke D;Beato M
虽然普遍存在于染色质中，但连接物组蛋白亚型的功能部分未知，关于它们的性质的相互矛盾的研究已经发表。为了探索不同的H1亚型在染色质的组织和动力学中是否具有不同的作用，我们将所有的体细胞H1亚型整合到微染色体中，并比较它们对核小体间距、染色质紧致和ATP依赖的重塑的影响。根据原子力显微镜的研究，H1亚型对染色质表现出不同的亲和力和促进染色质缩合的能力。根据这一标准，H1亚型可分为弱冷凝器(H1.1和H1.2)、中冷凝器(H1.3)和强冷凝器(H1.0、H1.4、H1.5和H1x)。如H1.4和H1.2之间的嵌合体所示，可变的C-末端结构域是核小体间距所必需的，并可能与各种亚型的染色质凝聚特性有关。与以前分离的核小体或线性核小体阵列的报道不同，每个核小体1的连接子组蛋白并不排除酵母SWI/SNF或果蝇NURF对微小染色体的重塑。我们假设连接物组蛋白亚型是染色质的不同组织者，而不是一般的抑制物。
Although ubiquitously present in chromatin, the function of the linker histone subtypes is partly unknown and contradictory studies on their properties have been published. To explore whether the various H1 subtypes have a differential role in the organization and dynamics of chromatin we have incorporated all of the somatic human H1 subtypes into minichromosomes and compared their influence on nucleosome spacing, chromatin compaction and ATP-dependent remodeling. H1 subtypes exhibit different affinities for chromatin and different abilities to promote chromatin condensation, as studied with the Atomic Force Microscope. According to this criterion, H1 subtypes can be classified as weak condensers (H1.1 and H1.2), intermediate condensers (H1.3) and strong condensers (H1.0, H1.4, H1.5 and H1x). The variable C-terminal domain is required for nucleosome spacing by H1.4 and is likely responsible for the chromatin condensation properties of the various subtypes, as shown using chimeras between H1.4 and H1.2. In contrast to previous reports with isolated nucleosomes or linear nucleosomal arrays, linker histones at a ratio of one per nucleosome do not preclude remodeling of minichromosomes by yeast SWI/SNF or Drosophila NURF. We hypothesize that the linker histone subtypes are differential organizers of chromatin, rather than general repressors.
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