Voltage imaging in the olfactory bulb using transgenic mouse lines expressing the genetically encoded voltage indicator ArcLight.
Voltage imaging in the olfactory bulb using transgenic mouse lines expressing the genetically encoded voltage indicator ArcLight.
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DOI：
10.1038/s41598-021-04482-3
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发表时间：
2022-02-03
影响因子：
4.6
通讯作者：
Storace DA
中科院分区：
文献类型：
--
作者：
Platisa J;Zeng H;Madisen L;Cohen LB;Pieribone VA;Storace DA
遗传编码电压指示器（GEVI）允许光学记录确定的细胞群体中的膜电位变化。促进具有高表达水平的精确和可重复靶向的转基因报告动物将进一步在体内哺乳动物脑中使用GEVI。然而，开发和应用转基因小鼠品系作为GEVI表达载体的文献是有限的。在这里，我们报告的第一个体内实验使用转基因报告小鼠的GEVI ArcLight，它利用了Cre/tTA依赖的表达系统（TIGRE 1.0）。我们开发了两种小鼠品系，其ArcLight表达仅限于嗅觉受体神经元或位于嗅球颗粒和肾小球层中的中间神经元亚群。这些细胞系中的ArcLight表达足以在使用落射荧光和2光子成像的单次试验中对气味反应进行体内成像。电压反应是气味特异性和浓度依赖性的，这支持了早期关于灯泡使用神经活动的钙传感器进行感知转换的研究。这项研究表明，ArcLight转基因系是一种灵活的遗传工具，可用于记录不同细胞类型的神经元电活动，其信噪比与以前使用病毒转导的报道相当。
Genetically encoded voltage indicators (GEVIs) allow optical recordings of membrane potential changes in defined cell populations. Transgenic reporter animals that facilitate precise and repeatable targeting with high expression levels would further the use of GEVIs in the in vivo mammalian brain. However, the literature on developing and applying transgenic mouse lines as vehicles for GEVI expression is limited. Here we report the first in vivo experiments using a transgenic reporter mouse for the GEVI ArcLight, which utilizes a Cre/tTA dependent expression system (TIGRE 1.0). We developed two mouse lines with ArcLight expression restricted to either olfactory receptor neurons, or a subpopulation of interneurons located in the granule and glomerular layers in the olfactory bulb. The ArcLight expression in these lines was sufficient for in vivo imaging of odorant responses in single trials using epifluorescence and 2-photon imaging. The voltage responses were odor-specific and concentration-dependent, which supported earlier studies about perceptual transformations carried out by the bulb that used calcium sensors of neural activity. This study demonstrates that the ArcLight transgenic line is a flexible genetic tool that can be used to record the neuronal electrical activity of different cell types with a signal-to-noise ratio that is comparable to previous reports using viral transduction.
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影响因子：
3.7
作者：
Dimitrov D;He Y;Mutoh H;Baker BJ;Cohen L;Akemann W;Knöpfel T
通讯作者：
Knöpfel T
影响因子：
3
作者：
Ma, MH;Chen, WR;Shepherd, GM
通讯作者：
Shepherd, GM
影响因子：
16.2
作者：
Jin L;Han Z;Platisa J;Wooltorton JR;Cohen LB;Pieribone VA
通讯作者：
Pieribone VA
影响因子：
5.3
作者：
Kohwi, Minoree;Petryniak, Magdalena A.;Alvarez-Buylla, Arturo
通讯作者：
Alvarez-Buylla, Arturo
影响因子：
48
作者：
Kannan M;Vasan G;Huang C;Haziza S;Li JZ;Inan H;Schnitzer MJ;Pieribone VA
通讯作者：
Pieribone VA