Hitchhiker's Guide to Voltammetry: Acute and Chronic Electrodes for in Vivo Fast-Scan Cyclic Voltammetry.
Hitchhiker's Guide to Voltammetry: Acute and Chronic Electrodes for in Vivo Fast-Scan Cyclic Voltammetry.
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Hitchhiker的伏安法指南：体内快速扫描循环伏安法的急性和慢性电极。
DOI：
10.1021/acschemneuro.6b00393
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发表时间：
2017-02-15
影响因子：
5
通讯作者：
Wightman RM
中科院分区：
文献类型：
--
作者：
Rodeberg NT;Sandberg SG;Johnson JA;Phillips PE;Wightman RM
快速扫描循环伏安法（FSCV）已被用于研究清醒和行为动物的快速神经传递超过20年。这些实验首先用硼硅酸盐玻璃包裹的碳纤维微电极（CFM）进行，它可以通过显微操作器和引导套管插入大脑。最近，已经引入了用小直径熔融二氧化硅构造的长期植入式CFM。这些电极可以以最小的组织反应固定在大脑中，这允许在行为期间在单个记录位置中纵向测量神经传递。这两种电极设计已被用于神经生物学、行为神经科学和精神药理学领域的新发现。本综述的目的是解决使用FSCV研究清醒和行为动物的神经递质的重要考虑因素，重点是纹状体多巴胺的测量。有关实验设计，数据收集和校准的常见问题得到解决。必要时，两种方法（急性与慢性记录）之间的差异进行了讨论。在这篇评论中提出的主题是特别重要的，因为该领域超越多巴胺向新的神经化学物质和大脑区域发展。
Fast-scan cyclic voltammetry (FSCV) has been used for over 20 years to study rapid neurotransmission in awake and behaving animals. These experiments were first carried out with carbon-fiber microelectrodes (CFMs) encased in borosilicate glass, which can be inserted into the brain through micromanipulators and guide cannulas. More recently, chronically implantable CFMs constructed with small diameter fused-silica have been introduced. These electrodes can be affixed in the brain with minimal tissue response, which permits longitudinal measurements of neurotransmission in single recording locations during behavior. Both electrode designs have been used to make novel discoveries in the fields of neurobiology, behavioral neuroscience, and psychopharmacology. The purpose of this Review is to address important considerations for the use of FSCV to study neurotransmitters in awake and behaving animals, with a focus on measurements of striatal dopamine. Common issues concerning experimental design, data collection, and calibration are addressed. When necessary, differences between the two methodologies (acute vs chronic recordings) are discussed. The topics raised in this Review are particularly important as the field moves beyond dopamine toward new neurochemicals and brain regions.
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影响因子：
56.9
作者：
EWING, AG;BIGELOW, JC;WIGHTMAN, RM
通讯作者：
WIGHTMAN, RM
影响因子：
48
作者：
Clark, Jeremy J.;Sandberg, Stefan G.;Wanat, Matthew J.;Gan, Jerylin O.;Horne, Eric A.;Hart, Andrew S.;Akers, Christina A.;Parker, Jones G.;Willuhn, Ingo;Martinez, Vicente;Evans, Scott B.;Stella, Nephi;Phillips, Paul E. M.
通讯作者：
Phillips, Paul E. M.
DOI：
10.1523/jneurosci.5119-12.2013
发表时间：
2013-02-20
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Clark JJ;Collins AL;Sanford CA;Phillips PE
通讯作者：
Phillips PE
影响因子：
16.2
作者：
Cheer, Joseph F.;Aragona, Brandon J.;Wightman, R. Mark
通讯作者：
Wightman, R. Mark
影响因子：
64.8
作者：
ARMSTRONGJAMES, M;MILLAR, J;KRUK, ZL
通讯作者：
KRUK, ZL