Caerin 1 Antimicrobial Peptides That Inhibit HIV and Neisseria May Spare Protective Lactobacilli.
Caerin 1 Antimicrobial Peptides That Inhibit HIV and Neisseria May Spare Protective Lactobacilli.
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抑制HIV和奈瑟菌的Caerin 1抗菌肽可能会保护乳酸菌。
DOI：
10.3390/antibiotics9100661
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发表时间：
2020-09-30
期刊：
影响因子：
--
通讯作者：
Reinert LK
中科院分区：
文献类型：
--
作者：
Rollins-Smith LA;Smith PB;Ledeczi AM;Rowe JM;Reinert LK
尽管由人类免疫缺陷病毒 (HIV) 引起的获得性免疫缺陷综合症 (AIDS) 对许多人来说是一种可以控制的疾病，但它仍然是许多其他人严重发病和经济困难的根源。艾滋病毒/艾滋病的主要传播方式是性交，需要采取措施减少传播。此前，我们发现源自澳大利亚两栖动物的caerin 1抗菌肽（AMP）可以在相对较低的浓度下抑制HIV的体外传播，并且对T细胞和宫颈内膜细胞系具有较低的毒性。使用 AMP 作为杀菌制剂的一部分会使阴道微生物组暴露于这些制剂，并对保护性乳杆菌造成潜在伤害。在这里，我们测试了 caerin 1 肽及其类似物对两种常见阴道乳杆菌（鼠李糖乳杆菌和卷曲乳杆菌）活力的影响。几种候选肽在抑制艾滋病毒的浓度范围内对乳酸菌具有有限的毒性。还测试了三种 AMP 抑制乳酸奈瑟菌（性传播淋病奈瑟菌的近亲）生长的能力。乳酸奈瑟菌对 AMP 的敏感性明显高于乳酸杆菌。因此，几种候选 AMP 具有抑制 HIV 和可能的淋病奈瑟菌传播的能力，其浓度对体内乳酸杆菌的危害明显较小。
Although acquired immunodeficiency syndrome (AIDS) caused by the human immunodeficiency virus (HIV) is a manageable disease for many, it is still a source of significant morbidity and economic hardship for many others. The predominant mode of transmission of HIV/AIDS is sexual intercourse, and measures to reduce transmission are needed. Previously, we showed that caerin 1 antimicrobial peptides (AMPs) originally derived from Australian amphibians inhibited in vitro transmission of HIV at relatively low concentrations and had low toxicity for T cells and an endocervical cell line. The use of AMPs as part of microbicidal formulations would expose the vaginal microbiome to these agents and cause potential harm to protective lactobacilli. Here, we tested the effects of caerin 1 peptides and their analogs on the viability of two species of common vaginal lactobacilli (Lactobacillus rhamnosus and Lactobacillus crispatus). Several candidate peptides had limited toxicity for the lactobacilli at a range of concentrations that would inhibit HIV. Three AMPs were also tested for their ability to inhibit growth of Neisseria lactamica, a close relative of the sexually transmissible Neisseria gonorrhoeae. Neisseria lactamica was significantly more sensitive to the AMPs than the lactobacilli. Thus, several candidate AMPs have the capacity to inhibit HIV and possible N. gonorrhoeae transmission at concentrations that are significantly less harmful to the resident lactobacilli.
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