Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis.
Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis.
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DOI：
10.1371/journal.pone.0010197
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发表时间：
2010-04-15
期刊：
影响因子：
3.7
通讯作者：
Fredricks DN
中科院分区：
文献类型：
--
作者：
Srinivasan S;Liu C;Mitchell CM;Fiedler TL;Thomas KK;Agnew KJ;Marrazzo JM;Fredricks DN
人们对人类阴道中细菌的短期波动知之甚少。本研究使用PCR评估健康女性中关键阴道细菌浓度的变化以及细菌性阴道病（BV）女性对抗生素治疗的即时反应。使用Amsel标准评估BV的22名妇女每天进行评估，持续7或14天，然后在第2、3和4周，使用一组11种细菌特异性定量PCR检测。BV患者阴道内注射甲硝唑5天。没有BV的参与者阴道分泌物以乳酸杆菌为主，其水平随月经波动。随着月经的开始，詹氏乳杆菌和卷曲乳杆菌的数量减少，并发现与阴道加德纳菌浓度呈负相关（p<0.001）。BV患者体内有多种苛养菌，在开始甲硝唑治疗后1-5天，其浓度降至检测阈值以下。复发性BV的特征是治疗后BV相关细菌最初显著减少，随后复发时增加。人类阴道的微生物群可以是高度动态的。健康的女性被乳酸杆菌种定殖，但水平可以在一个月内发生显着变化。在G.在月经期间观察迷走神经。BV患者体内有多种难养细菌群落，这些细菌通过阴道甲硝唑治疗而被消灭。复发性BV的女性最初对抗生素治疗有反应，细菌浓度急剧下降，但这些细菌后来重新出现，表明这些细菌的抗生素耐药性不是介导BV复发的重要因素。
Little is known about short-term bacterial fluctuations in the human vagina. This study used PCR to assess the variability in concentrations of key vaginal bacteria in healthy women and the immediate response to antibiotic treatment in women with bacterial vaginosis (BV). Twenty-two women assessed for BV using Amsel's criteria were evaluated daily for 7 or 14 days, then at 2, 3 and 4 weeks, using a panel of 11 bacterium-specific quantitative PCR assays. Participants with BV were treated with 5 days of intravaginal metronidazole. Participants without BV had vaginal biotas dominated by lactobacilli, whose levels fluctuated with menses. With onset of menstruation, quantities of Lactobacillus jensenii and Lactobacillus crispatus decreased and were found to be inversely related to Gardnerella vaginalis concentrations (p<0.001). Women with BV had a variety of fastidious bacteria whose concentrations dropped below detection thresholds 1–5 days after starting metronidazole. Recurrent BV was characterized by initial profound decreases of BV-associated bacteria after treatment followed by subsequent increases at relapse. The microbiota of the human vagina can be highly dynamic. Healthy women are colonized with Lactobacillus species, but levels can change dramatically over a month. Marked increases in G. vaginalis were observed during menses. Participants with BV have diverse communities of fastidious bacteria that are depleted by vaginal metronidazole therapy. Women with recurrent BV initially respond to antibiotic treatment with steep declines in bacterial concentrations, but these bacteria later reemerge, suggesting that antibiotic resistance in these bacteria is not an important factor mediating BV recurrence.
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