Identification and analysis of flagellar coexpressed determinants (Feds) of Campylobacter jejuni involved in colonization.
Identification and analysis of flagellar coexpressed determinants (Feds) of Campylobacter jejuni involved in colonization.
复制标题
DOI：
10.1111/j.1365-2958.2012.08027.x
复制
发表时间：
2012-04
影响因子：
3.6
通讯作者：
Hendrixson DR
中科院分区：
文献类型：
--
作者：
Barrero-Tobon AM;Hendrixson DR
空肠弯曲杆菌（Campylobacter jejuni）的鞭毛为鸟类肠道的共生定植和人类感染提供必要的动力，导致腹泻疾病。此外，据报道鞭毛III型分泌系统可分泌CiaI等蛋白，影响人肠细胞的侵袭并可能致病。鞭毛调控系统最终影响FlaA主鞭毛蛋白和其他鞭毛丝蛋白表达所需的σ28活性。在这项工作中，我们发现ciaI和四个我们建议注释为fed（鞭毛共表达决定因子）的基因的转录依赖于σ28，但这些基因不是运动所必需的。相反，联邦调查局和CiaI参与了小鸡的共生定植，联邦调查局还参与了促进人类肠道细胞的入侵。我们还发现，主要鞭毛蛋白影响σ28依赖性蛋白的产生、稳定性或分泌。确定了影响CiaI的特定转录和翻译机制，并分析了CiaI的结构域在共生或入侵中的重要性。我们的工作拓宽了鞭毛调控系统控制的基因，并暗示该系统与鞭毛协调定殖和毒力决定因素的产生，这些决定因素共同是与不同宿主进行最佳相互作用所必需的。
The flagellum of Campylobacter jejuni provides motility essential for commensal colonization of the intestinal tract of avian species and infection of humans resulting in diarrheal disease. Additionally, the flagellar type III secretion system has been reported to secrete proteins such as CiaI that influence invasion of human intestinal cells and possibly pathogenesis. The flagellar regulatory system ultimately influences σ28 activity required for expression of the FlaA major flagellin and other flagellar filament proteins. In this work, we discovered that transcription of ciaI and four genes we propose annotating as feds (for flagellar co-expressed determinants) is dependent upon σ28, but these genes are not required for motility. Instead, the Feds and CiaI are involved in commensal colonization of chicks, with FedA additionally involved in promoting invasion of human intestinal cells. We also discovered that the major flagellin influences production, stability or secretion of σ28-dependent proteins. Specific transcriptional and translational mechanisms affecting CiaI were identified and domains of CiaI were analyzed for importance in commensalism or invasion. Our work broadens the genes controlled by the flagellar regulatory system and implicates this system in coordinating production of colonization and virulence determinants with flagella, which together are required for optimal interactions with diverse hosts.
登录
查看更多内容
影响因子：
3.6
作者：
Christensen JE;Pacheco SA;Konkel ME
通讯作者：
Konkel ME
影响因子：
3.2
作者：
Kalmokoff, Martin;Lanthier, Patricia;Szymanski, Christine M.
通讯作者：
Szymanski, Christine M.
DOI：
10.1073/pnas.1113013108
发表时间：
2011-12-13
影响因子：
11.1
作者：
Boll, Joseph M.;Hendrixson, David R.
通讯作者：
Hendrixson, David R.
影响因子：
3.2
作者：
Konkel, ME;Klena, JD;Mickelson, J
通讯作者：
Mickelson, J
影响因子：
3.6
作者：
Hendrixson, DR;DiRita, VJ
通讯作者：
DiRita, VJ