Co-expression and co-purification of archaeal and eukaryal box C/D RNPs.
Co-expression and co-purification of archaeal and eukaryal box C/D RNPs.
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DOI：
10.1371/journal.pone.0103096
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Peng Y;Yu G;Tian S;Li H
Box C/D ribonucleoprotein particles (RNPs) are 2′-O-methylation enzymes required for maturation of ribosomal and small nuclear RNA. Previous biochemical and structural studies of the box C/D RNPs were limited by the unavailability of purified intact RNPs. We developed a bacterial co-expression strategy based on the combined use of a multi-gene expression system and a tRNA-scaffold construct that allowed the expression and purification of homogeneous archaeal and human box C/D RNPs. While the co-expressed and co-purified archaeal box C/D RNP was found to be fully active in a 2′-O-methylation assay, the intact human U14 box C/D RNP showed no detectable catalytic activity, consistent with the earlier findings that assembly of eukaryotic box C/D RNPs is nonspontaneous and requires additional protein factors. Our systems provide a means for further biochemical and structural characterization of box C/D RNPs and their assembly factors.
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DOI：
10.1126/science.1176099
发表时间：
2009-09-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bleichert F;Gagnon KT;Brown BA 2nd;Maxwell ES;Leschziner AE;Unger VM;Baserga SJ
通讯作者：
Baserga SJ
影响因子：
14.9
作者：
Ponchon L;Catala M;Seijo B;El Khouri M;Dardel F;Nonin-Lecomte S;Tisné C
通讯作者：
Tisné C
影响因子：
6.1
作者：
Liang, Bo;Li, Hong
通讯作者：
Li, Hong
DOI：
10.1083/jcb.200708110
发表时间：
2008-02-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Boulon S;Marmier-Gourrier N;Pradet-Balade B;Wurth L;Verheggen C;Jády BE;Rothé B;Pescia C;Robert MC;Kiss T;Bardoni B;Krol A;Branlant C;Allmang C;Bertrand E;Charpentier B
通讯作者：
Charpentier B
影响因子：
64.5
作者：
Balakin, AG;Smith, L;Fournier, MJ
通讯作者：
Fournier, MJ