Beyond D-luciferin: expanding the scope of bioluminescence imaging in vivo.

Beyond D-luciferin: expanding the scope of bioluminescence imaging in vivo.
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DOI:
10.1016/j.cbpa.2014.07.003
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发表时间:
2014-08
影响因子:
7.8
通讯作者:
Miller, Stephen C.
Miller, Stephen C.
中科院分区:
生物学2区
文献类型:
--
作者:
Adams, Spencer T., Jr.;Miller, Stephen C.

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The light-emitting chemical reaction catalyzed by the enzyme firefly luciferase is widely used for noninvasive imaging in live mice. However, photon emission from the luciferase is critically dependent on the chemical properties of its substrate, D-luciferin. In this review, we describe recent work to replace the natural luciferase substrate with synthetic analogs that extend the scope of bioluminescence imaging.
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