Engineering edgeless human skin with enhanced biomechanical properties.
Engineering edgeless human skin with enhanced biomechanical properties.
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DOI：
10.1126/sciadv.ade2514
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发表时间：
2023-01-27
期刊：
影响因子：
13.6
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中科院分区：
文献类型：
--
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尽管皮肤生物工程取得了进步，但3D皮肤构建体仍然被生产为具有开放边缘的平坦组织，而忽略了人类皮肤的完全封闭的几何形状。因此，它们不能有效地覆盖解剖学上复杂的身体部位，手在这里，我们通过将皮肤设计为完全封闭的3D组织来挑战流行的范式，该组织可以根据身体部位进行塑形，并作为生物服装无缝移植。与传统结构相比，我们的可穿戴无边缘皮肤结构（WESC）显示出增强的真皮细胞外基质（ECM）沉积和机械性能。WESC显示区域特异性细胞/ECM对齐，以及生理各向异性机械特性。WESC替代具有挑战性的身体部位的全层伤口中的皮肤（例如，小鼠后肢），具有最小的损伤和更短的手术时间。这项研究提供了一种引人注目的技术，可以大大改善伤口护理，并表明组织宏观解剖结构的重演可以增强生物功能。人类皮肤是生物工程作为一个可穿戴的组织与解剖形状定制完美匹配受体网站。
Despite the advancements in skin bioengineering, 3D skin constructs are still produced as flat tissues with open edges, disregarding the fully enclosed geometry of human skin. Therefore, they do not effectively cover anatomically complex body sites, e.g., hands. Here, we challenge the prevailing paradigm by engineering the skin as a fully enclosed 3D tissue that can be shaped after a body part and seamlessly transplanted as a biological clothing. Our wearable edgeless skin constructs (WESCs) show enhanced dermal extracellular matrix (ECM) deposition and mechanical properties compared to conventional constructs. WESCs display region-specific cell/ECM alignment, as well as physiologic anisotropic mechanical properties. WESCs replace the skin in full-thickness wounds of challenging body sites (e.g., mouse hindlimbs) with minimal suturing and shorter surgery time. This study provides a compelling technology that may substantially improve wound care and suggests that the recapitulation of the tissue macroanatomy can lead to enhanced biological function. Human skin is bioengineered as a wearable tissue with anatomical shapes tailored to perfectly match recipient sites.
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