Ergosterol-targeting fusion antifungal peptide significantly increases the Verticillium wilt resistance of cotton.
Ergosterol-targeting fusion antifungal peptide significantly increases the Verticillium wilt resistance of cotton.
复制标题
麦角甾醇靶向融合抗真菌肽显着提高棉花对黄萎病的抗性
DOI：
10.1111/pbi.13517
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发表时间：
2021-05
影响因子：
13.8
通讯作者：
Fan Y
中科院分区：
文献类型：
--
作者：
Tong S;Yuan M;Liu Y;Li X;Jin D;Cheng X;Lin D;Ling H;Yang D;Wang Y;Mao A;Pei Y;Fan Y
增加抗真菌蛋白对真菌细胞特定成分的靶向能力，有可能提高其抗真菌活性，减少对非靶细胞的有害影响。为了获得有效的棉花黄萎病抗性基因，我们构建了几个融合基因，分别将真菌细胞壁或细胞膜上针对甲壳素、鞘磷脂或麦角甾醇的结合域与来自昆虫病原真菌球孢白僵菌的抗菌肽BbAFP1融合。融合基因在棉子叶中的瞬时表达表明，含有麦角甾醇结合区的BbAFP1：：ERBD融合肽比野生型BbAFP1和其他融合基因具有更好的抗病能力。获得了BbAFP1：：ERBD和BbAFP1转基因棉花，并通过Southern和Western blotting进行了验证。与转BbAFP1基因棉花相比，转BbAFP1：：ERBD基因棉花对大丽叶枯萎病菌具有较高的抗病能力，叶片病斑面积较小(0.07×cm2比0.16×cm2)，病情指数较低(23.9比34.5)。与野生型基因相比，转基因烟草过表达BbAFP1：：ERBD基因也显示出对大丽叶枯病的抗性增强。这些结果表明，构建针对真菌细胞的融合抗菌肽是获得新的抗病基因的有效策略，所获得的融合基因BbAFP1：：ERBD具有防御植物真菌病害的潜力。
Increasing the targeting ability of antifungal proteins towards specific components of fungal cells has the potential to improve their antifungal activity and reduce harmful effects to nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we constructed several fusion genes, in which binding domains targeting chitin, sphingolipid or ergosterol in the fungal cell wall or cell membrane were individually fused to the antifungal peptide BbAFP1 from entomopathogenic fungus Beauveria bassiana. Transient expression of fusion genes in cotton cotyledons indicated that the BbAFP1::ErBD fusion peptide with an ergosterol binding domain exhibited better disease resistance against V. dahliae than wild‐type BbAFP1 and other fusion genes. BbAFP1::ErBD and BbAFP1 transgenic cotton were obtained and verified by Southern and Western blotting. Compared with BbAFP1‐expressing cotton, BbAFP1::ErBD‐expressing cotton showed higher disease resistance against V. dahliae, with smaller lesion areas (0.07 cm2 vs. 0.16 cm2) on the leaves and a lower disease index (23.9 vs. 34.5). Overexpression of BbAFP1::ErBD by transgenic tobacco also showed enhanced disease resistance against V. dahliae compared with that of the wild‐type gene. These results indicated that construction of fusion antifungal peptides that target fungal cells is a powerful strategy to obtain new anti‐disease genes, and the obtained fusion gene BbAFP1::ErBD has the potential to defend against plant fungal diseases.
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影响因子：
5.3
作者：
Gong Q;Yang Z;Wang X;Butt HI;Chen E;He S;Zhang C;Zhang X;Li F
通讯作者：
Li F
影响因子：
2.9
作者：
BROEKAERT, WF;MARIEN, W;CAMMUE, BPA
通讯作者：
CAMMUE, BPA
影响因子：
2.8
作者：
De Lucca, AJ;Bland, JM;Walsh, TJ
通讯作者：
Walsh, TJ
影响因子：
11.4
作者：
Gao, Wei;Long, Lu;Zhu, Longfu
通讯作者：
Zhu, Longfu
影响因子：
3.4
作者：
Ownley, Bonnie H.;Griffin, Mary R.;Pereira, Roberto M.
通讯作者：
Pereira, Roberto M.