Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.
Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.
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DOI：
10.1038/ncomms12808
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发表时间：
2016-09-20
影响因子：
16.6
通讯作者：
Kunieda, Takekazu
中科院分区：
文献类型：
--
作者：
Hashimoto, Takuma;Horikawa, Daiki D.;Saito, Yuki;Kuwahara, Hirokazu;Kozuka-Hata, Hiroko;Shin-, Tadasu, I;Minakuchi, Yohei;Ohishi, Kazuko;Motoyama, Ayuko;Aizu, Tomoyuki;Enomoto, Atsushi;Kondo, Koyuki;Tanaka, Sae;Hara, Yuichiro;Koshikawa, Shigeyuki;Sagara, Hiroshi;Miura, Toru;Yokobori, Shin-ichi;Miyagawa, Kiyoshi;Suzuki, Yutaka;Kubo, Takeo;Oyama, Masaaki;Kohara, Yuji;Fujiyama, Asao;Arakawa, Kazuharu;Katayama, Toshiaki;Toyoda, Atsushi;Kunieda, Takekazu
水熊虫，也被称为水熊，是小型水生动物。有些缓步动物可以忍受几乎完全的脱水，并在脱水状态下对各种物理极端表现出非凡的耐受性。在这里，我们确定了一个高质量的基因组序列Ramazzottius varieornatus，最耐胁迫的缓步动物物种之一。精确的基因库分析揭示了一小部分（1.2%或更少）的推定外源基因的存在下，损失的基因途径，促进应力损伤，扩大相关的基因家族改善损害，和进化和高表达的新缓步动物独特的蛋白质。在脱水和再水化过程中基因表达谱的微小变化表明耐受性相关基因的组成型表达。使用人类培养细胞，我们证明缓步动物独特的DNA相关蛋白可将X射线诱导的DNA损伤抑制0.40%，并提高辐射耐受性。这些发现表明缓步动物特有的蛋白质与耐受性的相关性，缓步动物可能是新的保护基因和机制的邦蒂富尔来源。 水熊虫能抵抗极端的环境条件，包括脱水、辐射和太空真空。在这里，作者提出了一个高质量的基因组，显示最小的水平基因转移，并确定了独特的缓步动物蛋白Dsup抑制DNA损伤。
Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by ∼40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms. Tardigrades are resistant to extreme environmental conditions including dehydration, radiation and the vacuum of space. Here the authors present a high-quality genome which displays minimal horizontal gene transfer, and identify the unique tardigrade protein Dsup which suppresses DNA damage.
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影响因子：
4.2
作者：
Horikawa, Daiki D.;Kunieda, Takekazu;Okuda, Takashi
通讯作者：
Okuda, Takashi
影响因子：
3.7
作者：
Horikawa DD;Cumbers J;Sakakibara I;Rogoff D;Leuko S;Harnoto R;Arakawa K;Katayama T;Kunieda T;Toyoda A;Fujiyama A;Rothschild LJ
通讯作者：
Rothschild LJ
Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta
DOI：
10.1038/nrm3522
发表时间：
2013-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
14.9
作者：
UniProt Consortium
通讯作者：
UniProt Consortium