Study of local hydrodynamic environment in cell-substrate adhesion using side-view μPIV technology.
Study of local hydrodynamic environment in cell-substrate adhesion using side-view μPIV technology.
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DOI：
10.1371/journal.pone.0030721
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Dong C
中科院分区：
文献类型：
--
作者：
Fu Y;Kunz R;Wu J;Dong C
在剪切流条件下肿瘤细胞粘附到内皮是导致循环介导的肿瘤转移的关键步骤。本研究提出了实验和计算技术，用于研究粘附细胞周围的局部流体动力学环境，以及局部剪切条件如何影响肿瘤细胞粘附内皮细胞上的细胞-细胞相互作用。为了研究异型贴壁细胞周围的局部流体动力学特性，建立了一种结合微粒子成像测速技术（μPIV）的侧视流动室实验，研究了近内皮壁区域白细胞与肿瘤细胞之间的相互作用和局部剪切流环境。还使用计算流体动力学（CFD）模拟来获得这些贴壁细胞周围的定量流动特性。结果表明，细胞尺寸和细胞间的相对位置对局部剪切速率有很大的影响。白细胞和肿瘤细胞上方的速度分布显示出非常不同的模式。较大的单元变形导致对流动的扰动较小。局部剪切速率以上较小的细胞观察到更受两个细胞之间的相对位置。
Tumor cell adhesion to the endothelium under shear flow conditions is a critical step that results in circulation-mediated tumor metastasis. This study presents experimental and computational techniques for studying the local hydrodynamic environment around adherent cells and how local shear conditions affect cell-cell interactions on the endothelium in tumor cell adhesion. To study the local hydrodynamic profile around heterotypic adherent cells, a side-view flow chamber assay coupled with micro particle imaging velocimetry (μPIV) technique was developed, where interactions between leukocytes and tumor cells in the near-endothelial wall region and the local shear flow environment were characterized. Computational fluid dynamics (CFD) simulations were also used to obtain quantitative flow properties around those adherent cells. Results showed that cell dimension and relative cell-cell positions had strong influence on local shear rates. The velocity profile above leukocytes and tumor cells displayed very different patterns. Larger cell deformations led to less disturbance to the flow. Local shear rates above smaller cells were observed to be more affected by relative positions between two cells.
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