Meta‐analyses of environmental DNA downstream transport and deposition in relation to hydrogeography in riverine environments
Meta‐analyses of environmental DNA downstream transport and deposition in relation to hydrogeography in riverine environments
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与河流环境水文地理相关的环境 DNA 下游运输和沉积的荟萃分析
DOI：
10.1111/fwb.13920
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发表时间：
2022
影响因子：
2.7
通讯作者：
Yamanaka Hiroki
中科院分区：
文献类型：
--
作者：
Jo Toshiaki;Yamanaka Hiroki
环境DNA（eDNA）分析是一种很有前途的工具，监测物种分布和丰富的水生生态系统。这些估计可能是有偏见的，在lotic环境中的eDNA运输和沉积过程的影响。然而，我们对eDNA下游运输动力学的理解是有限的，因为它可以波动很大，这取决于目标物种的丰度和eDNA的数量在一个源站点和水生采样的设计。此外，eDNA沉积到底部基底中也决定了水性eDNA的可用性，但关于沉积的知识要有限得多。因此，有关于eDNA下游传输和沉积，以及它们与水文地理conditions.In这项研究中，我们整理了以前的论文有关河流eDNA传输和合成有关eDNA传输和沉积的知识。一些研究最近应用了流体动力学建模方法，并计算了eDNA下游运输的平均长度（Sw）和eDNA沉积的速度（Vdep）。参考这些研究，我们从9篇eDNA论文中手动计算或直接提取SwandVdep，并进行Meta分析，以揭示这些估计值与河流流量的关联。在此基础上，模拟了给定水文地理条件下eDNA向下游输送的平均长度，估算的SwandVdep值分别为2.6 ~ 13187.4m和0.003 ~ 1.900mm/s。对数转换后，中位SwandVdep值为102.188（= 154.1）m和10-1.387（= 0.041）mm/s。直到95%和99%的靶eDNA颗粒沉积，计算的最大eDNA运输距离分别为334.0至1，272.7 m和513.5至1，956.4 m。此外，我们发现，河流排放量呈正相关与Sw，whereasVdepis低于自然环境比围隔。这种趋势可能是由于其他环境因素，如底部基质。我们的Meta分析表明，在普通的水文地理条件下，即使考虑到来自河床的eDNA再悬浮，大多数eDNA颗粒也可以向下游移动不到2 km。相比之下，我们的研究没有考虑可能影响eDNA转运的多种环境因素，因此会在研究中引入中度到高度的异质性。这些问题将在未来的研究中得到解决。据我们所知，这项研究是第一个定量合成的长度在河流环境中的eDNA下游运输，并推断eDNA下游运输距离和沉积速度的一般属性。根据我们的结果，个体释放的大部分eDNA可能对下游2公里以上采样点目标eDNA的检测影响不大。虽然还需要进一步积累经验研究，但我们的研究结果为河流生态系统中最佳eDNA采样设计奠定了基础，以减少物种存在的假阳性推断和假阴性eDNA检测。
Environmental DNA (eDNA) analysis is a promising tool to monitor species distribution and abundance in aquatic ecosystems. These estimates can be biased in lotic environments by the effects of eDNA transport and deposition processes. However, our understanding of eDNA downstream transport dynamics is limited because it can fluctuate greatly depending on target species abundance and eDNA quantity in a source site and the design of aquatic sampling. Furthermore, eDNA deposition into bottom substrates also determines the availability of aqueous eDNA, but knowledge about deposition is much more limited. Consequently, there is little consensus about eDNA downstream transport and deposition, as well as their interactions with hydrogeographic conditions.In this study, we compiled previous papers concerning riverine eDNA transport and synthesised knowledge about eDNA transport and deposition. A few studies have recently applied a hydrodynamic modelling approach and calculated the average length of eDNA downstream transport (Sw) and velocity of eDNA deposition (Vdep). Referring to those studies, we manually calculated, or directly extracted,SwandVdepfrom the nine eDNA papers and conducted meta‐analyses to reveal the association of these estimates with river discharges. Furthermore, we simulated the mean length of eDNA downstream transport in a channel with given hydrogeographic conditions.The estimatedSwandVdepvalues ranged from 2.6 to 13,187.4 m and 0.003 to 1.900 mm/s, respectively. When log‐transformed, the medianSwandVdepvalues were 102.188(= 154.1) m and 10–1.387(= 0.041) mm/s. The calculated maximum eDNA transport distances until 95% and 99% of the target eDNA particles are deposited ranged from 334.0 to 1,272.7 m and 513.5 to 1,956.4 m, respectively. Moreover, we found that river discharges were positively associated withSw, whereasVdepwas lower for natural environments than mesocosms. This trend could be due to other environmental factors, such as bottom substrates.Our meta‐analysis implied that, under ordinary hydrogeographic conditions, most eDNA particles could travel less than 2 km downstream even when considering the resuspension of eDNA from riverbeds. By contrast, our study did not consider multiple environmental factors that potentially affect eDNA transport and would accordingly introduce a moderate to high degree of heterogeneity across studies. These issues will be addressed in future studies.To our knowledge, this study is the first to quantitatively synthesise the length of eDNA downstream transport in riverine environments and infer general properties of eDNA downstream transport distance and deposition velocity. According to our results, much of the eDNA released from individuals might have little effect on the detection of target eDNA in the sampling sites more than 2 km downstream. Although further accumulation of empirical studies is necessary, our findings propose the groundwork for optimal eDNA sampling designs in riverine ecosystems to reduce the false‐positive inference of species presence and false‐negative eDNA detection.
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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Schwartz, Michael K.
贻贝环境 DNA 的河流分布反映了密度、运输和清除过程之间的平衡
影响因子：
2.7
作者：
Arial J. Shogren;J. Tank;S. Egan;D. Bolster;T. Riis
通讯作者：
T. Riis
DOI：
10.1016/j.scitotenv.2017.06.255
发表时间：
2017-12
期刊：
The Science of the total environment
影响因子：
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作者：
Jeffery W. Song;M. Small;Elizabeth A. Casman
通讯作者：
Jeffery W. Song;M. Small;Elizabeth A. Casman