Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae.
Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae.
复制标题
NMD的失活增加了酿酒酵母中Sup45废话突变体的生存能力。
DOI：
10.1186/1471-2199-8-71
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发表时间：
2007-08-16
影响因子：
--
通讯作者：
Zhouravleva, Galina
中科院分区：
文献类型：
--
作者：
Chabelskaya, Svetlana;Gryzina, Valentina;Moskalenko, Svetlana;Le Goff, Catherine;Zhouravleva, Galina
无意义介导的mRNA衰变(NMD)途径促进含有提前终止密码子(PTCs)的mRNAs的快速降解。在酿酒酵母中，NMD途径的活性依赖于翻译机制对PTC的识别。翻译终止因子eRF1(Sup45)和eRF3(Sup35)不仅参与蛋白质合成的最后一步，而且通过与Pab1、UPF1、Upf2和Upf3等蛋白质相互作用，参与mRNA的降解和翻译起始。在这项工作中，我们使用以前分离的酿酒酵母su45突变体来表征当翻译终止受损的条件下异常mRNA的降解。我们已经测定了his7-1、lys9-a21和trp1-289等位基因的序列，这些等位基因是分析无意义抑制的常用等位基因。我们已经证实，sup45无义和错义突变会导致His7-1mRNA和CYH2前-mRNA的积聚。值得注意的是，UPF1基因的缺失抑制了一些超45表型。其中，超45-nUPF1Δ双突变体对温度的敏感性低于超45单突变体，对帕罗霉素的抗性更强。此外，UPF2或UPF3的缺失恢复了sup45-n双突变体的活性。这是首次证明，sup45突变不仅改变了翻译的保真度，而且还通过引起mRNA稳定性的改变而起作用。
The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature termination codons (PTCs). In yeast Saccharomyces cerevisiae, the activity of the NMD pathway depends on the recognition of the PTC by the translational machinery. Translation termination factors eRF1 (Sup45) and eRF3 (Sup35) participate not only in the last step of protein synthesis but also in mRNA degradation and translation initiation via interaction with such proteins as Pab1, Upf1, Upf2 and Upf3. In this work we have used previously isolated sup45 mutants of S. cerevisiae to characterize degradation of aberrant mRNA in conditions when translation termination is impaired. We have sequenced his7-1, lys9-A21 and trp1-289 alleles which are frequently used for analysis of nonsense suppression. We have established that sup45 nonsense and missense mutations lead to accumulation of his7-1 mRNA and CYH2 pre-mRNA. Remarkably, deletion of the UPF1 gene suppresses some sup45 phenotypes. In particular, sup45-n upf1Δ double mutants were less temperature sensitive, and more resistant to paromomycin than sup45 single mutants. In addition, deletion of either UPF2 or UPF3 restored viability of sup45-n double mutants. This is the first demonstration that sup45 mutations do not only change translation fidelity but also acts by causing a change in mRNA stability.
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影响因子：
10.5
作者：
Czaplinski, K;Ruiz-Echevarria, MJ;Peltz, SW
通讯作者：
Peltz, SW
影响因子：
10.5
作者：
HE, F;JACOBSON, A
通讯作者：
JACOBSON, A
影响因子：
10.5
作者：
LEEDS, P;PELTZ, SW;CULBERTSON, MR
通讯作者：
CULBERTSON, MR
影响因子：
5.6
作者：
ALTAMURA, N;GROUDINSKY, O;SLONIMSKI, PP
通讯作者：
SLONIMSKI, PP
影响因子：
64.8
作者：
Amrani, N;Ganesan, R;Jacobson, A
通讯作者：
Jacobson, A