Two bases are deleted from the termini of HIV-1 linear DNA during integrative recombination.
Two bases are deleted from the termini of HIV-1 linear DNA during integrative recombination.
复制标题
在整合重组过程中，HIV-1 线性 DNA 的末端有两个碱基被删除。
DOI：
10.1016/0042-6822(90)90161-j
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Pauza,CD
中科院分区：
文献类型：
--
作者：
Pauza,CD
前病毒整合是逆转录病毒生命周期中必需的步骤。整合机制涉及线性病毒DNA末端的特异性修饰以及随后与宿主序列的重组。整合导致病毒基因组末端序列信息的有限丢失。通过对线性分子进行分子内平端连接时形成的 2 长末端重复 (2-LTR) 环状连接进行测序，推断出完整线性 DNA 末端的组成。连接序列包含整合原病毒末端不存在的核苷酸 GTAC。与HIV-1的LAV-1株的序列比较表明，来自线性病毒DNA的右手末端(U5)的GT二核苷酸和来自左手末端(U3)的AC二核苷酸。因此，LAV-1 LTR 的校正大小为 637 bp。通过评估急性感染 T 细胞中线性病毒 DNA 的结构，独立证实了这一结论。线性 HIV-1 DNA 分子群体的一部分在其 3' 端被特异性删除；该缺失的范围是2个碱基。该结果与线性病毒 DNA 上病毒整合酶蛋白的活性一致，并且它解释了整合的 HIV-1 原病毒的结构。
Proviral integration is a required step in the retrovirus life cycle. The mechanism of integration involves specific modification of the ends of linear viral DNA and subsequent recombination with host sequences. Integration results in the limited loss of sequence information at the termini of the viral genome. The composition of the intact linear DNA termini were inferred by sequencing the 2-long terminal repeat (2-LTR) circle junction that is formed when the linear molecule undergoes intramolecular, blunt-end ligation. The junction sequence contained the nucleotides GTAC that were not present at the ends of the integrated provirus. Comparison with the sequence of the LAV-1 strain of HIV-1 demonstrated that the GT dinucleotide derived from the right-hand terminus (U5) of the linear viral DNA and the AC dinucleotide came from the left-hand terminus (U3). Therefore, the corrected size of the LAV-1 LTR is 637 bp. This conclusion was confirmed independently by assessing the structure of linear viral DNA in acutely infected T cells. A portion of the population of linear HIV-1 DNA molecules were specifically deleted at their 3′ ends; the extent of this deletion was 2 bases. This result is consistent with the activity of viral intergrase protein on linear viral DNA and it accounts for the structure of integrated HIV-1 proviruses.
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影响因子：
64.5
作者：
FUJIWARA, T;MIZUUCHI, K
通讯作者：
MIZUUCHI, K
逆转录期间有序链间和链内 DNA 转移。
影响因子：
56.9
作者：
A. Panganiban;D. Fiore
通讯作者：
D. Fiore
影响因子：
5.4
作者：
COOPER, GM;TEMIN, HM
通讯作者：
TEMIN, HM
影响因子：
64.5
作者：
BATTULA, N;TEMIN, HM
通讯作者：
TEMIN, HM
影响因子：
64.5
作者：
WAINHOBSON, S;SONIGO, P;ALIZON, M
通讯作者：
ALIZON, M