A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge.
A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge.
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DOI：
10.3390/md19020105
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发表时间：
2021-02-11
期刊：
影响因子：
5.4
通讯作者：
Race PR
中科院分区：
文献类型：
--
作者：
Back CR;Stennett HL;Williams SE;Wang L;Ojeda Gomez J;Abdulle OM;Duffy T;Neal C;Mantell J;Jepson MA;Hendry KR;Powell D;Stach JEM;Essex-Lopresti AE;Willis CL;Curnow P;Race PR
为了解决日益严重的抗生素耐药性问题，必须确定新的生物活性化合物，这些化合物可有效对抗耐药微生物并安全使用。天然产物及其衍生物是，并将继续是这些分子的重要来源。海绵中含有与海绵共存的多种微生物组，这些细菌群落产生丰富的生物活性代谢物，用于保护和资源竞争。由于这些原因，海绵微生物群构成了临床相关天然产物的潜在来源。迄今为止，对这些化合物进行生物勘探的努力主要集中在从浅水中分离出的海绵标本上，关于深海的样本还有很多有待了解。在这里，我们报告了一种新的小单孢菌菌株的分离，命名为28 ISP 2 - 46 T，从大西洋中部深海海绵的微生物组中回收。全基因组测序揭示了这种细菌产生多种天然产物的能力，包括表现出抗生素和抗肿瘤特性的kosinostatin和异喹环素B。这两种化合物都是从28 ISP 2 - 46 T发酵液中分离出来的，并且被发现对过多的多药耐药临床分离株有效。这项研究表明，海洋生产的异喹环素可能比以前设想的更广泛，并证明了将深海海绵微生物组作为具有可开发的生物合成潜力的新型微生物生命来源的价值。
To tackle the growing problem of antibiotic resistance, it is essential to identify new bioactive compounds that are effective against resistant microbes and safe to use. Natural products and their derivatives are, and will continue to be, an important source of these molecules. Sea sponges harbour a diverse microbiome that co-exists with the sponge, and these bacterial communities produce a rich array of bioactive metabolites for protection and resource competition. For these reasons, the sponge microbiota constitutes a potential source of clinically relevant natural products. To date, efforts in bioprospecting for these compounds have focused predominantly on sponge specimens isolated from shallow water, with much still to be learned about samples from the deep sea. Here we report the isolation of a new Micromonospora strain, designated 28ISP2-46T, recovered from the microbiome of a mid-Atlantic deep-sea sponge. Whole-genome sequencing reveals the capacity of this bacterium to produce a diverse array of natural products, including kosinostatin and isoquinocycline B, which exhibit both antibiotic and antitumour properties. Both compounds were isolated from 28ISP2-46T fermentation broths and were found to be effective against a plethora of multidrug-resistant clinical isolates. This study suggests that the marine production of isoquinocyclines may be more widespread than previously supposed and demonstrates the value of targeting the deep-sea sponge microbiome as a source of novel microbial life with exploitable biosynthetic potential.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5.2
作者：
Konig, Christian M.;Harms, Klaus;Koert, Ulrich
通讯作者：
Koert, Ulrich
影响因子：
3.6
作者：
Gerwick, William H.;Fenner, Amanda M.
通讯作者：
Fenner, Amanda M.
影响因子：
4.6
作者：
Carro L;Nouioui I;Sangal V;Meier-Kolthoff JP;Trujillo ME;Montero-Calasanz MDC;Sahin N;Smith DL;Kim KE;Peluso P;Deshpande S;Woyke T;Shapiro N;Kyrpides NC;Klenk HP;Göker M;Goodfellow M
通讯作者：
Goodfellow M
影响因子：
6.1
作者：
Intra, Bungonsiri;Greule, Anja;Panbangred, Watanalai
通讯作者：
Panbangred, Watanalai