Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species.
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species.
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DOI：
10.3390/ani12213052
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发表时间：
2022-11-06
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影响因子：
--
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中科院分区：
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--
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许多微小的(微)染色体是鸟类的一个特征，在其他生物中发现的数量较少，在许多生物中没有，如哺乳动物。虽然微染色体构成了鸟类基因组的很大一部分，但有关它们的数据仍然很少，用于鸟类之间的比较研究。滨鸟就是这种情况，这是一个物种种类繁多的类群。本研究的目的是深入了解三种滨鸟--红结滨鸟(Calidris Canutus)、有节滨鸟(Jacana Jacana)和南翅滨鸟(Vanellus Chilensis)的微染色体进化。这些实验被称为跨物种荧光原位杂交(FISH)，使用被称为细菌人工染色体(BAC)的探针，每个微染色体有两个(一个用红色标记，一个用绿色标记)。因此，结果显示为一端为绿色和一端为红色的微染色染色体，揭示了进化过程中不同的组织模式。在红结中，它们融合在一起，但在南方，它们几乎没有变化。我们还描述了一个新的红结染色体数目(总共92个)。综上所述，本研究有助于了解三种滨鸟的微染色体组织和进化规律。微染色体曾经被认为是基因组中不重要的元素，但它们是鸟类核型的基本组成部分。滨鸟(Charadriiformes)种类繁多，约有390个物种，被认为是生物学研究的有价值的模式组。尽管有这种多样性，但在这种鸟类目中，细胞遗传学分析仍然非常稀少。因此，这项研究的目的是提供对Charadriiformes核型的洞察，重点是结合经典和分子方法对三种滨鸟-Calidris canutus、Jacana Jacana和Vanellus Chilens的微染色体进化。跨物种FISH定位在每个微染色体上应用了两个BAC探针，GGA10-28(GGA16除外)。这些实验揭示了所研究物种中不同的微染色体组织模式。因此，虽然我们在加拿大华支睾吸虫中发现了参与染色体融合的两条微染色体，但在雪莲中它们是以单对的形式存在的。我们还描述了一个新的斜纹夜蛾染色体数目(2n=92)。因此，本研究有助于了解三种滨鸟的基因组组织和进化。
Numerous tiny (micro)chromosomes are a characteristic feature associated with birds, being found in smaller numbers in other organisms and absent in many, such as mammals. Although microchromosomes constitute a large portion of the genome in birds, data on them pertaining to comparative studies between birds are still scarce. This is the case in shorebirds (Charadriiformes), a group with a great variety of species. The aim of this study was to provide insight regarding the evolution of the microchromosomes of three species of shorebirds—the red knot (Calidris canutus), the wattled jacana (Jacana jacana), and the southern lapwing (Vanellus chilensis). The experiments are referred to as cross-species fluorescence in situ hybridization (FISH) mapping using probes called bacterial artificial chromosomes (or BACs), two (one labelled in red and one labelled in green) for every microchromosome. The results thus appear as the microchrochromosome with one green and one red end, revealing different patterns of organization over evolutionary time. In the red knot, they fuse together, but in the southern lapwing, they hardly change. We also described a new chromosome number for the red knot (92 in total). In conclusion, this study contributed to the understanding of microchromosomes organization and evolution of three shorebird species. Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds—Calidris canutus, Jacana jacana, and Vanellus chilensis—combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C. canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V. chilensis. We also described a new chromosome number for C. canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species.
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影响因子：
6
作者：
Kretschmer R;Gunski RJ;Garnero ADV;de Freitas TRO;Toma GA;Cioffi MB;Oliveira EHC;O'Connor RE;Griffin DK
通讯作者：
Griffin DK
影响因子：
6
作者：
Kiazim LG;O'Connor RE;Larkin DM;Romanov MN;Narushin VG;Brazhnik EA;Griffin DK
通讯作者：
Griffin DK
影响因子：
30.8
作者：
Kuepper, Clemens;Stocks, Michael;Risse, Judith E.;dos Remedios, Natalie;Farrell, Lindsay L.;Mcrae, Susan B.;Morgan, Tawna C.;Karlionova, Natalia;Pinchuk, Pavel;Verkuil, Yvonne I.;Kitaysky, Alexander S.;Wingfield, John C.;Piersma, Theunis;Zeng, Kai;Slate, Jon;Blaxter, Mark;Lank, David B.;Burke, Terry
通讯作者：
Burke, Terry
影响因子：
1.7
作者：
Griffin, D. K.;Robertson, L. B. W.;Skinner, B. M.
通讯作者：
Skinner, B. M.
影响因子：
2.4
作者：
Joseph, Sunitha;O'Connor, Rebecca E.;Griffin, Darren K.
通讯作者：
Griffin, Darren K.