Newly discovered ebola virus associated with hemorrhagic fever outbreak in Uganda.
Newly discovered ebola virus associated with hemorrhagic fever outbreak in Uganda.
复制标题
新发现的与乌干达出血热爆发有关的埃博拉病毒。
DOI：
10.1371/journal.ppat.1000212
复制
发表时间：
2008-11
期刊：
影响因子：
6.7
通讯作者：
Nichol, Stuart T.
中科院分区：
文献类型：
--
作者：
Towner, Jonathan S.;Sealy, Tara K.;Khristova, Marina L.;Albarino, Cesar G.;Conlan, Sean;Reeder, Serena A.;Quan, Phenix-Lan;Lipkin, W. Ian;Downing, Robert;Tappero, Jordan W.;Okware, Samuel;Lutwama, Julius;Bakamutumaho, Barnabas;Kayiwa, John;Comer, James A.;Rollin, Pierre E.;Ksiazek, Thomas G.;Nichol, Stuart T.
在过去的30年里，扎伊尔和苏丹埃博拉病毒是造成大规模出血热（HF）暴发的原因，病死率从53%到90%不等，而第三个物种科特迪瓦埃博拉病毒引起了一例非致命性HF病例。2007年11月，乌干达西部本迪布焦县报告了HF病例。通过经典方法（抗原捕获、IgM和IgG ELISA）和最近开发的随机引物焦磷酸测序方法对最初的29份疑似病例血液标本进行实验室调查，迅速确定这是一次埃博拉HF疫情，与一种新发现的埃博拉病毒物种（本迪布焦埃博拉病毒）有关，该病毒与西非发现的科特迪瓦埃博拉病毒有远亲关系。由于这种新病毒相对于所有以前认识到的埃博拉病毒的序列差异，这些发现对设计未来的诊断检测方法以监测人类和动物中的埃博拉HF疾病以及正在进行的开发有效的抗病毒药物和疫苗的努力具有重要意义。在这份报告中，我们描述了一个新发现的埃博拉病毒物种，造成了大规模的出血热疫情在乌干达西部。该病毒在遗传上是不同的，在基因组水平上与所有其他已知的埃博拉病毒物种的差异超过30%。这种病毒的独特性质对传统的基于丝状病毒分子的诊断测定和基因组测序方法提出了挑战。相反，我们使用最近开发的随机引物焦磷酸测序方法快速确定了超过70%的病毒基因组，该方法允许快速开发用于疾病爆发响应的分子检测方法。该序列草案允许使用传统的引物步移方法轻松完成全基因组序列，并迅速确认该病毒代表一种新的埃博拉病毒物种。目前设计有效的诊断、抗病毒和疫苗的努力需要考虑到丝状病毒家族这一重要新成员的独特性质。
Over the past 30 years, Zaire and Sudan ebolaviruses have been responsible for large hemorrhagic fever (HF) outbreaks with case fatalities ranging from 53% to 90%, while a third species, Côte d'Ivoire ebolavirus, caused a single non-fatal HF case. In November 2007, HF cases were reported in Bundibugyo District, Western Uganda. Laboratory investigation of the initial 29 suspect-case blood specimens by classic methods (antigen capture, IgM and IgG ELISA) and a recently developed random-primed pyrosequencing approach quickly identified this to be an Ebola HF outbreak associated with a newly discovered ebolavirus species (Bundibugyo ebolavirus) distantly related to the Côte d'Ivoire ebolavirus found in western Africa. Due to the sequence divergence of this new virus relative to all previously recognized ebolaviruses, these findings have important implications for design of future diagnostic assays to monitor Ebola HF disease in humans and animals, and ongoing efforts to develop effective antivirals and vaccines. In this report we describe a newly discovered ebolavirus species which caused a large hemorrhagic fever outbreak in western Uganda. The virus is genetically distinct, differing by more than 30% at the genome level from all other known ebolavirus species. The unique nature of this virus created challenges for traditional filovirus molecular based diagnostic assays and genome sequencing approaches. Instead, we quickly determined over 70% of the virus genome using a recently developed random-primed pyrosequencing approach that allowed the rapid development of a molecular detection assay that was deployed in the disease outbreak response. This draft sequence allowed easy completion of the whole genome sequence using a traditional primer walking approach and prompt confirmation that this virus represented a new ebolavirus species. Current efforts to design effective diagnostics, antivirals and vaccines will need to take into account the distinct nature of this important new member of the filovirus family.
登录
查看更多内容
影响因子：
5.8
作者：
Nylander, Johan A. A.;Wilgenbusch, James C.;Swofford, David L.
通讯作者：
Swofford, David L.
影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
3.7
作者：
Towner JS;Pourrut X;Albariño CG;Nkogue CN;Bird BH;Grard G;Ksiazek TG;Gonzalez JP;Nichol ST;Leroy EM
通讯作者：
Leroy EM
影响因子：
6.4
作者：
Ksiazek, TG;West, CP;Peters, CJ
通讯作者：
Peters, CJ
影响因子：
6.4
作者：
Ksiazek, TG;Rollin, PE;Peters, CJ
通讯作者：
Peters, CJ