Multiple factors and processes involved in host cell killing by bacteriophage Mu: characterization and mapping.
Multiple factors and processes involved in host cell killing by bacteriophage Mu: characterization and mapping.
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噬菌体 Mu 杀伤宿主细胞涉及的多种因素和过程：表征和作图。
DOI：
10.1016/0042-6822(84)90257-5
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Pato,ML
中科院分区：
文献类型：
--
作者：
Waggoner,BT;Marrs,CF;Howe,MM;Pato,ML
通过从左端开始携带不同数量Mu DNA的12个λ pmu转导噬菌体感染λ免疫宿主，确定噬菌体Mu参与宿主细胞杀伤的区域。感染含有5.0（±0.2）kb或更少的Mu DNA左端的λpMu噬菌体不会杀死λ免疫宿主，而含有5.1 kb的λpMu噬菌体可以杀死λ免疫宿主，从而将kilgene的右端定位在大约5.0和5.1 kb之间。对于Kil+噬菌体，杀伤程度随着感染的多重性（m.o.i.）的增加而增加。此外，至少有两个其他区域的存在也会影响到杀戮：位于5.1 - 5.8 kb之间的一个区域降低了杀戮程度；另一个位于6.3 ~ 7.9 kb之间，可大大增加宿主细胞的杀伤。λpMu感染具有λ免疫功能的宿主后，也检测了该宿主的杀伤作用，该宿主携带了大部分bgene和Mu DNA中部缺失的mini-Mu。无论是否存在Mukilgene，通过感染B+λpMu噬菌体来补充mini-Mu复制，都可以杀死具有λ免疫的、含有mini-Mu的宿主。宿主细胞的杀伤程度随着感染λpMu的m.o.i的增加而增加，并且kilgene和位于6.3 ~ 7.9 kb之间的区域的存在进一步增强了宿主细胞的杀伤程度。在诱导复制缺陷型和杀伤突变型前噬菌体后，分别观察到在没有复制的情况下，杀伤介导的杀伤和在有复制的情况下，非杀伤介导的杀伤的不同过程。
The regions of bacteriophage Mu involved in host cell killing were determined by infection of a λ-immune host with 12 λpMu-transducing phages carrying different amounts of Mu DNA beginning at the left end. Infecting λpMu phages containing 5.0 (±0.2) kb or less of the left end of Mu DNA did not kill the λ-immune host, whereas λpMu containing 5.1 kb did kill, thus locating the right end of thekilgene between approximately 5.0 and 5.1 kb. For the Kil+phages the extent of killing increased as the multiplicity of infection (m.o.i.) increased. In addition, killing was also affected by the presence of at least two other regions of Mu DNA: one, located between 5.1 and 5.8 kb, decreased the extent of killing; the other, located between 6.3 and 7.9 kb, greatly increased host cell killing. Killing was also assayed after λpMu infection of a λ-immune host carrying a mini-Mu deleted for most of theBgene and the middle region of Mu DNA. Complementation of mini-Mu replication by infecting B+λpMu phages resulted in killing of the λ-immune, mini-Mu-containing host, regardless of the presence or absence of the Mukilgene. The extent of host cell killing increased as the m.o.i. of the infecting λpMu increased, and was further enhanced by both the presence of thekilgene and the region located between 6.3 and 7.9 kb. These distinct processes ofkil-mediated killing in the absence of replication and non-kil-mediated killing in the presence of replication were also observed after induction of replication-deficient andkilmutant prophages, respectively.
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影响因子：
3.7
作者：
Marrs,CF;Howe,MM
通讯作者：
Howe,MM
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.7
作者：
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通讯作者：
P. Van de Putte
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影响因子：
3.7
作者：
K. O'Day;D. Schultz;W. Ericsen;L. Rawluk;M. Howe
通讯作者：
M. Howe
影响因子：
56.9
作者：
BLATTNER, FR;WILLIAMS, BG;SMITHIES, O
通讯作者：
SMITHIES, O