Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
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DOI：
10.1016/j.brainresbull.2006.12.009
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发表时间：
2007-04-02
影响因子：
3.8
通讯作者：
Lowry CA
中科院分区：
文献类型：
--
作者：
Bouwknecht JA;Spiga F;Staub DR;Hale MW;Shekhar A;Lowry CA
源自中缝尾核和尾中缝背核（DR）的血清素能系统与焦虑药物或厌恶刺激引起的焦虑相关行为反应的促进有关。在这项研究中，我们试图确定在焦虑相关行为测试中暴露于厌恶条件后，DR 中血清素能神经元参与的阈值。在对雄性 Wistar 大鼠的研究中，我们通过让它们不受干扰 (CO)、短暂地处理它们 (HA) 或在低光 (LL: 8-13 lux) 或高光 (HL: 400-500 lux) 条件下将它们暴露在开放场地 15 分钟来控制致焦虑刺激的强度。与 LL 大鼠相比，暴露于 HL 条件的大鼠的反应是运动活动减少、探索竞技场中心的时间减少、饲养和梳理毛发的频率较低以及面对竞技场角落的频率增加。暴露于 HL 条件的大鼠在 DR 喙部的 5-羟色胺能神经元内 c-Fos 表达有小幅但显着的增加。暴露于 HL 条件不会改变任何其他 DR 分支中血清素能神经元的 c-Fos 反应。相反，与 CO 大鼠相比，暴露于开放场地的大鼠在整个 DR 期间非血清素能细胞中的 c-Fos 表达增加，并且这种效应在 DR 的背外侧部分尤其明显。我们得出的结论是，与 LL 条件相比，暴露于 HL 条件会增加开放场地中与焦虑相关的行为反应，但这种刺激等于或低于增加 5-羟色胺能神经元中 c-Fos 表达所需的阈值。
Serotonergic systems arising from the mid-rostrocaudal and caudal dorsal raphe nucleus (DR) have been implicated in the facilitation of anxiety-related behavioral responses by anxiogenic drugs or aversive stimuli. In this study we attempted to determine a threshold to engage serotonergic neurons in the DR following exposure to aversive conditions in an anxiety-related behavioral test. We manipulated the intensity of anxiogenic stimuli in studies of male Wistar rats by leaving them undisturbed (CO), briefly handling them (HA), or exposing them to an open-field arena for 15-min under low-light (LL: 8-13 lux) or high-light (HL: 400-500 lux) conditions. Rats exposed to HL conditions responded with reduced locomotor activity, reduced time spent exploring the center of the arena, a lower frequency of rearing and grooming, and an increased frequency of facing the corner of the arena compared to LL rats. Rats exposed to HL conditions had small but significant increases in c-Fos expression within serotonergic neurons in subdivisions of the rostral DR. Exposure to HL conditions did not alter c-Fos responses in serotonergic neurons in any other DR subdivision. In contrast, rats exposed to the open-field arena had increased c-Fos expression in non-serotonergic cells throughout the DR compared to CO rats, and this effect was particularly apparent in the dorsolateral part of the DR. We conclude that exposure to HL conditions, compared to LL conditions, increased anxiety-related behavioral responses in an open-field arena but this stimulus was at or below the threshold required to increase c-Fos expression in serotonergic neurons.
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