The Nrf2a pathway impacts zebrafish offspring development with maternal preconception exposure to perfluorobutanesulfonic acid.
The Nrf2a pathway impacts zebrafish offspring development with maternal preconception exposure to perfluorobutanesulfonic acid.
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DOI：
10.1016/j.chemosphere.2021.132121
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发表时间：
2022-01
期刊：
影响因子：
8.8
通讯作者：
Timme-Laragy AR
中科院分区：
文献类型：
--
作者：
Annunziato KM;Marin M;Liang W;Conlin SM;Qi W;Doherty J;Lee J;Clark JM;Park Y;Timme-Laragy AR
自自愿淘汰全氟辛烷磺酸以来，全氟丁烷磺酸等较小的同系物已被用作工业替代品，并在受污染的含水层中被检测到。本研究旨在研究母体孕前PFBS暴露对卵子和健康后代发育的影响。成年雌性斑马鱼接受了为期一周的0.08，0.14和0.25毫克/升PFBS的水生暴露。之后，将雌性与未暴露的雄性交配，并在5次成功的交配事件中收集胚胎。在第一次采集中检测到PFBS浓度范围为99-253皮克/胚胎，但在第四次和第五次采集时低于定量限。因此，随后将数据分组为检测到PFBS的早期采集胚胎和PFBS低于定量的晚期采集胚胎。在早期收集中，胚胎24小时存活率显著降低。在后期收集中，胚胎发育受到Nrf 2a野生型和突变幼虫之间出现的独特模式的影响。此外，通过测量脂肪酸谱、总胆固醇和甘油三酯含量来评估胚胎营养负荷的影响。没有明确的剂量依赖性效应，但在基因型之间再次观察到独特的模式。研究发现，妊娠前PFBS暴露会改变卵子和胚胎发育，这是由卵子中的直接毒物负荷、卵子中的营养负荷和Nrf 2a的功能介导的。这些研究结果提供了深入了解PFBS的生殖和发育影响，并确定母亲的先入之见作为一个新的关键窗口的曝光。
Since the voluntary phaseout of perfluorooctanesulfonic acid (PFOS), smaller congeners, such as perfluorobutanesulfonic acid (PFBS) have served as industrial replacements and been detected in contaminated aquifers. This study sought to examine the effects of a maternal preconception PFBS exposure on the development of eggs and healthy offspring. Adult female zebrafish received a one-week waterborne exposure of 0.08, 0.14, and 0.25 mg/L PFBS. After which, females were bred with non-exposed males and embryos collected over 5 successful breeding events. PFBS concentrations were detected in levels ranging from 99–253 pg/embryo in the first collection but were below the limit of quantitation by fourth and fifth clutches. Therefore, data were subsequently binned into early collection embryos in which PFBS was detected and late collections, in which PFBS was below quantitation. In the early collection, embryo 24 hr survival was significantly reduced. In the late collection, embryo development was impacted with unique patterns emerging between Nrf2a wildtype and mutant larvae. Additionally, the impact of nutrient loading into the embryos was assessed through measurement of fatty acid profiles, total cholesterol, and triglyceride content. There were no clear dose-dependent effects, but again unique patterns were observed between the genotypes. Preconception PFBS exposures were found to alter egg and embryo development, which is mediated by direct toxicant loading in the eggs, nutrient loading into eggs, and the function of Nrf2a. These findings provide insight into the reproductive and developmental effects of PFBS and identify maternal preconception as a novel critical window of exposure.
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影响因子：
5.9
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通讯作者：
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影响因子：
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