Innovative high-resolution microCT imaging of animal brain vasculature.
Innovative high-resolution microCT imaging of animal brain vasculature.
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动物脑血管的创新高分辨率microCT成像。
DOI：
10.1007/s00429-020-02158-8
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发表时间：
2020-12
影响因子：
3.1
通讯作者：
Djonov V
中科院分区：
文献类型：
--
作者：
Hlushchuk R;Haberthür D;Soukup P;Barré SF;Khoma OZ;Schittny J;Haghayegh Jahromi N;Bouchet A;Engelhardt B;Djonov V
分析导管血管和微血管的血管结构和定量分析对于理解中枢神经系统的生理和病理过程至关重要。大多数可用的体内成像方法缺乏穿透深度和/或分辨率。一些离体方法可能提供更好的分辨率，但主要是破坏性的，因为它们是设计用于从颅骨或脊柱中取出中枢神经系统组织后的成像。切除过程不可避免地改变了所研究结构的原位关系，损害了硬脑膜和脑膜。µAngiofil是一种基于聚合物的造影剂，允许一种定性新颖的死后微血管计算机断层扫描（微血管oct）方法，具有优异的分辨率，因此可以可视化最小的脑毛细血管。获得的数据集可以对血管树进行相当直接的定量分析，包括微血管系统。µAngiofil具有出色的填充能力以及比骨组织更高的放射不透明度，即使在完整的头骨或脊柱内也可以成像大脑微血管。这允许原位可视化，从而调查硬脑膜和薄脑膜层及其原始几何形状的血液供应。此外，这里介绍的方法允许相关的方法，即微血管oct，然后是经典组织学，免疫组织化学甚至电子显微镜。本文提出的实验方法利用了常见的桌面微ct扫描仪，使其成为临床前和基础研究中评估中枢神经系统（微）脉管系统的有前途的日常工具。本文的在线版本（10.1007/s00429-020-02158-8）包含补充资料，仅供授权用户使用。
Analysis of the angioarchitecture and quantification of the conduit vessels and microvasculature is of paramount importance for understanding the physiological and pathological processes within the central nervous system (CNS). Most of the available in vivo imaging methods lack penetration depth and/or resolution. Some ex vivo methods may provide better resolution, but are mainly destructive, as they are designed for imaging the CNS tissues after their removal from the skull or vertebral column. The removal procedure inevitably alters the in situ relations of the investigated structures and damages the dura mater and leptomeninges. µAngiofil, a polymer-based contrast agent, permits a qualitatively novel postmortem microangio-computed tomography (microangioCT) approach with excellent resolution and, therefore, visualization of the smallest brain capillaries. The datasets obtained empower a rather straightforward quantitative analysis of the vascular tree, including the microvasculature. The µAngiofil has an excellent filling capacity as well as a radio-opacity higher than the one of bone tissue, which allows imaging the cerebral microvasculature even within the intact skull or vertebral column. This permits in situ visualization and thus investigation of the dura mater and leptomeningeal layers as well as their blood supply in their original geometry. Moreover, the methodology introduced here permits correlative approaches, i.e., microangioCT followed by classical histology, immunohistochemistry and even electron microscopy. The experimental approach presented here makes use of common desktop microCT scanners, rendering it a promising everyday tool for the evaluation of the (micro)vasculature of the central nervous system in preclinical and basic research. The online version of this article (10.1007/s00429-020-02158-8) contains supplementary material, which is available to authorized users.
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影响因子：
2.8
作者：
Shi X
通讯作者：
Shi X
DOI：
10.1177/0271678x15606723
发表时间：
2016-02
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
Østergaard L;Engedal TS;Moreton F;Hansen MB;Wardlaw JM;Dalkara T;Markus HS;Muir KW
通讯作者：
Muir KW
影响因子：
48
作者：
Pan, Chenchen;Cai, Ruiyao;Ertuerk, Ali
通讯作者：
Ertuerk, Ali
影响因子：
3.2
作者：
Sengle, Gerhard;Tufa, Sara F.;Keene, Douglas R.
通讯作者：
Keene, Douglas R.
影响因子：
5.7
作者：
Demene, Charlie;Tiran, Elodie;Tanter, Mickael
通讯作者：
Tanter, Mickael