Ion mobility-mass spectrometry analysis of isomeric carbohydrate precursor ions.
Ion mobility-mass spectrometry analysis of isomeric carbohydrate precursor ions.
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DOI：
10.1007/s00216-009-2865-y
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发表时间：
2009-08
影响因子：
4.3
通讯作者：
Hill, Herbert H., Jr.
中科院分区：
文献类型：
--
作者：
Zhu, Maolei;Bendiak, Brad;Clowers, Brian;Hill, Herbert H., Jr.
使用与四极离子阱连接的常压离子迁移谱仪 (IMS) 证明了寡糖异构体前体离子在 MSn 分析之前的快速分离。分离不限于特定类型的异构体；仅在糖的立体化学、异头构型以及总体分支模式和连接位置方面不同的代表性异构体可以在毫秒时间范围内解析。前体离子的物理分离允许获得至少 MS3 的单个寡糖异构体的独立质谱，解离阶段的数量实际上仅受特定产物离子的丰度限制。 IMS-MSn 分析对于评估异构寡糖特别有价值，这些寡糖在 MS/MS 实验中产生相同的产物离子组，揭示了如果仅通过 MS 解离方法评估则无法得知混合物中存在的异构体对。描述了对从牛颌下粘蛋白释放的寡糖的单个 HPLC 级分中包含的一组异构体进行 IMS-MSn 分析的实际示例。
The rapid separation of isomeric precursor ions of oligosaccharides prior to their analysis by MSn was demonstrated using an ambient pressure ion mobility spectrometer (IMS) interfaced with a quadrupole ion trap. Separations were not limited to specific types of isomers; representative isomers differing solely in the stereochemistry of sugars, in their anomeric configurations, and in their overall branching patterns and linkage positions could be resolved in the millisecond time frame. Physical separation of precursor ions permitted independent mass spectra of individual oligosaccharide isomers to be acquired to at least MS3, the number of stages of dissociation limited only practically by the abundance of specific product ions. IMS-MSn analysis was particularly valuable in the evaluation of isomeric oligosaccharides that yielded identical sets of product ions in MS/MS experiments, revealing pairs of isomers that would otherwise not be known to be present in a mixture if evaluated solely by MS dissociation methods alone. A practical example of IMS-MSn analysis of a set of isomers included within a single HPLC fraction of oligosaccharides released from bovine submaxillary mucin is described.
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影响因子：
2.9
作者：
BENDIAK, B;HARRISBRANDTS, M;CUMMING, DA
通讯作者：
CUMMING, DA
DOI：
10.1016/j.jasms.2007.04.007
发表时间：
2007-07-01
影响因子：
3.2
作者：
Dwivedi, Prabha;Bendiak, Brad;Hill, Herbert H., Jr.
通讯作者：
Hill, Herbert H., Jr.
DOI：
10.1046/j.1432-1327.1998.2580603.x
发表时间：
1998-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Mårtensson, S;Levery, SB;Bendiak, B
通讯作者：
Bendiak, B
影响因子：
6.4
作者：
Sverremark, E;Fernandez, C
通讯作者：
Fernandez, C
影响因子：
5.3
作者：
Angata, Kiyohiko;Huckaby, Valerie;Fukuda, Minoru
通讯作者：
Fukuda, Minoru