Soft, strong, tough, and durable protein-based fiber hydrogels.
Soft, strong, tough, and durable protein-based fiber hydrogels.
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DOI：
10.1073/pnas.2213030120
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发表时间：
2023-02-21
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
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作者：
新兴的生物医学应用，如组织工程和人工组织，要求植入材料与活组织在生物学和机械学上相容。然而，这两种性能的组合在当前的材料系统中尚未实现。天然来源的材料具有组织的基本生物学特征，但往往是脆弱的。而合成聚合物可以复制组织的机械性能，但往往缺乏支持和指导活细胞的能力。在这里，我们提出了一个战略，以克服这一困境。我们使用蛋白质水凝胶（如明胶）模拟组织的层次结构。这种结构导致软组织样的机械性能，均匀的3D细胞化和巨大的生物活性的组合。承载软组织通常表现出J形应力-应变行为，在低应变下具有高顺应性，而在高应变下具有高强度。它们含水量高，但仍然坚韧耐用。相比之下，天然衍生的水凝胶是弱的和易碎的。虽然由合成聚合物制备的水凝胶可以是坚固和坚韧的，但它们不具有新兴生物医学应用所需的生物活性。在这里，我们提出了一种热机械的方法来复制的组合性质的软组织蛋白质为基础的光交联水凝胶。作为演示，我们制造了一种具有类似软组织机械性能的明胶甲基丙烯酰纤维水凝胶，例如低杨氏模量（0.1至0.3 MPa）、高强度（1.1 ± 0.2 MPa）、高韧性（9，100 ± 2，200 J/m3）和高抗疲劳性（2，300 ± 500 J/m2）。这种水凝胶还类似于天然细胞外基质的生物化学和结构特性，这使得水凝胶内部能够快速形成3D互连的细胞网络。纤维结构还调节细胞机械反应并支持水凝胶内的细胞重塑。组织样力学性能和生物活性的集成是下一代生物材料的高度期望，并且可以推进新兴领域，如组织工程和再生医学。
Emerging biomedical applications, such as tissue engineering and artificial tissues, require implant materials to be both biologically and mechanically compatible with living tissues. However, the combination of these two properties has not been achieved in current material systems. Naturally derived materials carry the essential biological features of tissues but tend to be weak and brittle. Whereas synthetic polymers can replicate the mechanical properties of tissues but often lack the ability to support and direct live cells. Here, we present a strategy to overcome this dilemma. We mimic the hierarchical structure of tissues using protein-based hydrogels such as gelatin. This structure leads to the combination of soft tissue-like mechanical properties, uniform 3D cellularization, and great bioactivity. Load-bearing soft tissues normally show J-shaped stress–strain behaviors with high compliance at low strains yet high strength at high strains. They have high water content but are still tough and durable. By contrast, naturally derived hydrogels are weak and brittle. Although hydrogels prepared from synthetic polymers can be strong and tough, they do not have the desired bioactivity for emerging biomedical applications. Here, we present a thermomechanical approach to replicate the combinational properties of soft tissues in protein-based photocrosslinkable hydrogels. As a demonstration, we create a gelatin methacryloyl fiber hydrogel with soft tissue-like mechanical properties, such as low Young’s modulus (0.1 to 0.3 MPa), high strength (1.1 ± 0.2 MPa), high toughness (9,100 ± 2,200 J/m3), and high fatigue resistance (2,300 ± 500 J/m2). This hydrogel also resembles the biochemical and architectural properties of native extracellular matrix, which enables a fast formation of 3D interconnected cell meshwork inside hydrogels. The fiber architecture also regulates cellular mechanoresponse and supports cell remodeling inside hydrogels. The integration of tissue-like mechanical properties and bioactivity is highly desirable for the next-generation biomaterials and could advance emerging fields such as tissue engineering and regenerative medicine.
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影响因子：
18.9
作者：
Han X;Alu A;Liu H;Shi Y;Wei X;Cai L;Wei Y
通讯作者：
Wei Y
影响因子：
18.9
作者：
Elkhoury K;Morsink M;Sanchez-Gonzalez L;Kahn C;Tamayol A;Arab-Tehrany E
通讯作者：
Arab-Tehrany E
影响因子：
41.2
作者：
Lee HP;Gu L;Mooney DJ;Levenston ME;Chaudhuri O
通讯作者：
Chaudhuri O
影响因子：
14
作者：
Liu, Bingcheng;Wang, Ying;Xing, Malcolm
通讯作者：
Xing, Malcolm
影响因子：
4.1
作者：
Bai, Ruobing;Yang, Jiawei;Suo, Zhigang
通讯作者：
Suo, Zhigang