Physiological and proteomic analyses on artificially aged Brassica napus seed.
Physiological and proteomic analyses on artificially aged Brassica napus seed.
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DOI：
10.3389/fpls.2015.00112
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发表时间：
2015
影响因子：
5.6
通讯作者：
Yang P
中科院分区：
文献类型：
--
作者：
Yin X;He D;Gupta R;Yang P
当植物种子经过长期储存或受控变质处理时，就会通过称为种子老化的过程失去活力。根据之前的研究，已经开发出人工老化处理来加速种子老化过程，以了解其潜在机制。在这项研究中，我们使用甘蓝型油菜种子来研究衰老起始机制。对甘蓝型油菜种子进行人工老化处理（40°C 和 90% 相对湿度），并分析其生理生化特性。尽管这种处理延迟了发芽，但它并没有增加细胞活性氧（ROS）的浓度。在不同发芽阶段的对照和处理种子之间进行比较蛋白质组分析。对治疗有反应的蛋白质主要涉及代谢、蛋白质修饰和目的地、应激反应、发育和杂酶。除过氧化还原酶外，人工老化种子中其他抗氧化酶的积累没有观察到变化。在人工处理的种子中观察到脱落酸（ABA）含量增加，这可能与抑制发芽有关。总而言之，我们的结果强调了除了 ROS 之外，ABA 还参与了种子老化的启动，而 ROS 之前被报道可以介导种子老化过程。
Plant seeds lose their viability when they are exposed to long term storage or controlled deterioration treatments, by a process known as seed aging. Based on previous studies, artificially aging treatments have been developed to accelerate the process of seed aging in order to understand its underlying mechanisms. In this study, we used Brassica napus seeds to investigate the mechanisms of aging initiation. B. napus seeds were exposed to artificially aging treatment (40°C and 90% relative humidity) and their physio-biochemical characteristics were analyzed. Although the treatment delayed germination, it did not increase the concentration of cellular reactive oxygen species (ROS). Comparative proteomic analysis was conducted among the control and treated seeds at different stages of germination. The proteins responded to the treatment were mainly involved in metabolism, protein modification and destination, stress response, development, and miscellaneous enzymes. Except for peroxiredoxin, no changes were observed in the accumulation of other antioxidant enzymes in the artificially aged seeds. Increased content of abscisic acid (ABA) was observed in the artificially treated seeds which might be involved in the inhibition of germination. Taken together, our results highlight the involvement of ABA in the initiation of seed aging in addition to the ROS which was previously reported to mediate the seed aging process.
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影响因子：
13.8
作者：
Lee J;Welti R;Roth M;Schapaugh WT;Li J;Trick HN
通讯作者：
Trick HN
影响因子：
7.4
作者：
Job, C;Rajjou, L;Job, D
通讯作者：
Job, D
影响因子：
7.2
作者：
Nakabayashi, K;Okamoto, M;Nambara, E
通讯作者：
Nambara, E
影响因子：
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作者：
Li, Ming;Sha, Aihua;Yang, Pingfang
通讯作者：
Yang, Pingfang
影响因子：
3.4
作者：
Chi, Feng;Yang, Pingfang;Shen, Shihua
通讯作者：
Shen, Shihua