The critical role of myeloperoxidase in Streptococcus pneumoniae clearance and tissue damage during mouse acute otitis media
The critical role of myeloperoxidase in Streptococcus pneumoniae clearance and tissue damage during mouse acute otitis media
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髓过氧化物酶在小鼠急性中耳炎期间肺炎链球菌清除和组织损伤中的关键作用
DOI：
10.1177/1753425917693907
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发表时间：
2017-04
期刊：
影响因子：
3.2
通讯作者：
He Yujuan
中科院分区：
文献类型：
--
作者：
Xiang Yun;Jin Chunfang;Wang Wei;Wang Zimeng;Huang Yifei;Fan Fangmei;Ma Yurong;Zhang Xuemei;Xu Wenchun;Yin Yibing;He Yujuan
我们最近报道，中性粒细胞在小鼠急性中耳炎(AOM)时对肺炎链球菌(Spn)的天然防御中起着关键作用。然而，潜在的机制仍不清楚。通过建立C57BL/6小鼠肺炎球菌AOM模型，并使用体内特异性抑制剂，研究中性粒细胞最重要的蛋白质组分之一髓过氧化物酶(MPO)的作用。实验结果表明，在Spn感染的小鼠中，招募的中性粒细胞MPO的产生显著增加。中性粒细胞以MPO依赖的方式杀死Spn。MPO促进了活性氧(ROS)的产生，从而促进了Spn的早期清除，加剧了组织损伤。此外，MPO可诱导中性粒细胞凋亡和坏死，进而加重组织损伤。综上所述，我们的研究表明，中性粒细胞MPO在肺炎球菌AOM的细菌清除和组织损伤中扮演着矛盾的角色。
We have recently reported that neutrophils play a pivotal role in innate defense against Streptococcus pneumoniae (Spn) during mouse acute otitis media (AOM). However, the underlying mechanism remains unclear. By constructing models of pneumococcal AOM in C57BL/6 mice and using a specific inhibitor in vivo, we investigated the role of myeloperoxidase (MPO), one of the most important protein components of neutrophils. Experiment results showed a significant increase in MPO production of the recruited neutrophils in Spn-infected mice. Neutrophils killed Spn in a MPO-dependent manner. MPO facilitated the generation of reactive oxygen species (ROS), and consequently promoted Spn clearance at an early stage and exacerbated tissue damage. Moreover, MPO induced neutrophil apoptosis and necrosis, which, in turn, worsened tissue damage. In summary, our study demonstrates that neutrophil MPO plays a paradoxical role in bacterial clearance and tissue damage in pneumococcal AOM.
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DOI：
10.1007/s10495-008-0187-8
发表时间：
2008-04
期刊：
Apoptosis : an international journal on programmed cell death
影响因子：
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DOI：
10.1177/0194599814525576
发表时间：
2014-08
期刊：
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影响因子：
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