A protein complex network of Drosophila melanogaster.
A protein complex network of Drosophila melanogaster.
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DOI：
10.1016/j.cell.2011.08.047
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发表时间：
2011-10-28
期刊：
影响因子：
64.5
通讯作者：
Artavanis-Tsakonas S
中科院分区：
文献类型：
--
作者：
Guruharsha KG;Rual JF;Zhai B;Mintseris J;Vaidya P;Vaidya N;Beekman C;Wong C;Rhee DY;Cenaj O;McKillip E;Shah S;Stapleton M;Wan KH;Yu C;Parsa B;Carlson JW;Chen X;Kapadia B;VijayRaghavan K;Gygi SP;Celniker SE;Obar RA;Artavanis-Tsakonas S
确定蛋白质复合体的组成是理解细胞作为一个综合系统的关键一步。使用共亲和纯化结合质谱分析，我们检查了涉及近5000个单独的FLAG-HA表位标记的果蝇蛋白的蛋白质关联性。基于一种新的统计框架来定义单个蛋白质-蛋白质相互作用，对这些数据的严格分析导致了包含556个蛋白质复合体的果蝇蛋白质相互作用图(DPIM)的产生。DPIM的高质量及其作为后生动物蛋白质组范例的有效性从许多已知复合体的恢复、共享功能属性的显著丰富以及在人类细胞中的验证中可见一斑。DPIM为几个重要的蛋白质复合体定义了潜在的新成员，并将功能链接分配给586个缺乏先前实验注释的蛋白质编码基因。据我们所知，DPIM代表了最大的后生动物蛋白质复合体图谱，为分析蛋白质复合体进化提供了宝贵的资源。
Determining the composition of protein complexes is an essential step towards understanding the cell as an integrated system. Using co-affinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly five thousand individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a novel statistical framework to define individual protein-protein interactions, led to the generation of a Drosophila Protein interaction Map (DPiM) encompassing 556 protein complexes. The high quality of DPiM and its usefulness as a paradigm for metazoan proteomes is apparent from the recovery of many known complexes, significant enrichment for shared functional attributes and validation in human cells. DPiM defines potential novel members for several important protein complexes and assigns functional links to 586 protein-coding genes lacking previous experimental annotation. DPiM represents, to our knowledge, the largest metazoan protein complex map and provides a valuable resource for analysis of protein complex evolution.
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影响因子：
4.4
作者：
Eng, Jimmy K.;Fischer, Bernd;MacCoss, Michael J.
通讯作者：
MacCoss, Michael J.
影响因子：
64.8
作者：
Gavin, AC;Aloy, P;Superti-Furga, G
通讯作者：
Superti-Furga, G
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE
影响因子：
3.8
作者：
GRAHAM, FL;SMILEY, J;NAIRN, R
通讯作者：
NAIRN, R
影响因子：
9.2
作者：
Freilich, S;Oron, E;Chamovitz, DA
通讯作者：
Chamovitz, DA