An expanding bacterial colony forms a depletion zone with growing droplets.
An expanding bacterial colony forms a depletion zone with growing droplets.
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DOI：
10.1039/d0sm01348j
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发表时间：
2021-03-04
期刊：
影响因子：
3.4
通讯作者：
Tang JX
中科院分区：
文献类型：
--
作者：
Ma H;Bell J;Chen W;Mani S;Tang JX
许多种类的细菌已经发展出在固体表面上传播的有效手段。本研究的重点是扩大铜绿假单胞菌在琼脂凝胶表面的条件下，最小的蒸发。我们报告的发生和扩展的菌落内的耗尽区的蔓延，在那里的细菌负载膜变得更薄。耗尽区与较高浓度的鼠李糖脂共定位，鼠李糖脂是由细菌产生并在殖民地的较老区域中积累的生物表面活性剂。随着种群的持续增长，密集的细菌液滴出现并在耗尽区聚结，显示出显著的流体动力学行为。尽管中心耗尽区的扩张需要活细菌的活动，但可以通过添加鼠李糖脂在其他地方接种新的区域。由于添加了表面活性剂，这些耗尽区迅速扩大，即使在被紫外线杀死的细菌覆盖的平板上也是如此。我们解释所观察到的性能的基础上考虑细菌的生长和分泌，渗透膨胀，流体体积膨胀，界面流体动力学，涉及马兰戈尼和毛细管流动，细胞-细胞凝聚力。
Many species of bacteria have developed effective means to spread on solid surfaces. This study focuses on the expansion of Pseudomonas aeruginosa on an agar gel surface under conditions of minimal evaporation. We report the occurrence and spread of a depletion zone within an expanded colony, where the bacteria laden film becomes thinner. The depletion zone is colocalized with a higher concentration of rhamnolipids, the biosurfactants that are produced by the bacteria and accumulate in the older region of the colony. With continued growth in population, dense bacterial droplets occur and coalesce in the depletion zone, displaying remarkable fluid dynamic behavior. Whereas expansion of a central depletion zone requires activities of live bacteria, new zones can be seeded elsewhere by adding rhamnolipids. These depletion zones due to the added surfactants expand quickly, even on plates covered by bacteria that have been killed by ultraviolet light. We explain the observed properties based on considerations of bacterial growth and secretion, osmotic swelling, fluid volume expansion, interfacial fluid dynamics involving Marangoni and capillary flows, and cell-cell cohesion.
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影响因子：
3.6
作者：
Boles, BR;Thoendel, M;Singh, PK
通讯作者：
Singh, PK
影响因子：
3.4
作者：
Fauvart, M.;Phillips, P.;Vermant, J.
通讯作者：
Vermant, J.
DOI：
10.1073/pnas.0910934107
发表时间：
2010-02-23
影响因子：
11.1
作者：
Butler, Mitchell T.;Wang, Qingfeng;Harshey, Rasika M.
通讯作者：
Harshey, Rasika M.
影响因子：
3.4
作者：
Darnton, Nicholas C.;Turner, Linda;Berg, Howard C.
通讯作者：
Berg, Howard C.
影响因子：
2.7
作者：
Allison, DG
通讯作者：
Allison, DG