The expression of superoxide dismutase (SOD) and a putative ABC transporter permease is inversely correlated during biofilm formation in Listeria monocytogenes 4b G.
The expression of superoxide dismutase (SOD) and a putative ABC transporter permease is inversely correlated during biofilm formation in Listeria monocytogenes 4b G.
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单核细胞增生李斯特菌 4b G 生物膜形成过程中超氧化物歧化酶 (SOD) 和假定的 ABC 转运蛋白通透酶的表达呈负相关
DOI：
10.1371/journal.pone.0048467
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Shi X
中科院分区：
文献类型：
--
作者：
Suo Y;Huang Y;Liu Y;Shi C;Shi X
目前对单核细胞增生李斯特菌生物膜形成的分子基础知之甚少。lm.G_1771基因缺失突变体的超氧化物歧化酶（SOD）在生物膜中高表达，该突变体编码一种推定的ABC转运通透酶。本研究对sod基因缺失突变体Δsod，以及sod和lm双缺失突变体。利用G_1771基因Δ1771Δsod研究SOD在生物被膜形成中的作用及其与ABC转运蛋白通透酶表达的关系。我们的研究结果表明，Δsod突变体和Δ1771Δsod双突变体形成生物膜的能力显著降低。Δsod和Δ1771Δsod突变体均表现出生长缓慢的表型，并产生更多的活性氧（ROS）。甲基紫精（MV，内氧自由基发生器）处理抑制突变体的生长。此外，分析了一个抗氧化基因（kat）、两个胁迫调节基因（perR和sigB）和一个DNA修复基因（recA）在两种野生型L.通过RT-qPCR检测单核细胞增多症4 b G和缺失突变体。当生物膜形成时，这四个基因在缺失突变体中的表达水平增加。两者合计，我们的数据表明，SOD通过应对氧化剂负担在缺乏抗氧化剂防御生物膜的形成中发挥了重要作用。
Little is known about the molecular basis of biofilm formation in Listeria monocytogenes. The superoxide dismutase (SOD) of the deletion mutant of lm.G_1771 gene, which encodes for a putative ABC transporter permease, is highly expressed in biofilm. In this study, the sod gene deletion mutant Δsod, and double deletion mutant of the sod and lm. G_1771 genes Δ1771Δsod were used to investigate the role of SOD and its relationship to the expression of the putative ABC transporter permease in biofilm formation. Our results showed that the ability to form a biofilm was significantly reduced in the Δsod mutant and the Δ1771Δsod double mutant. Both Δsod and Δ1771Δsod mutants exhibited slow growth phenotypes and produced more reactive oxygen species (ROS). The growth was inhibited in the mutants by methyl viologen (MV, internal oxygen radical generator) treatment. In addition, the expression of one oxidation resistance gene (kat), two stress regulators encoding genes (perR and sigB), and one DNA repair gene (recA) were analyzed in both the wild-type L. monocytogenes 4b G and the deletion mutants by RT-qPCR. The expression levels of the four genes were increased in the deletion mutants when biofilms were formed. Taken together, our data indicated that SOD played an important role in biofilm formation through coping with the oxidant burden in deficient antioxidant defenses.
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影响因子：
2.1
作者：
Bitoun JP;Nguyen AH;Fan Y;Burne RA;Wen ZT
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影响因子：
8.8
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Kuzminov, A
通讯作者：
Kuzminov, A
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通讯作者：
Hecker, M