ATL9, a RING zinc finger protein with E3 ubiquitin ligase activity implicated in chitin- and NADPH oxidase-mediated defense responses.
ATL9, a RING zinc finger protein with E3 ubiquitin ligase activity implicated in chitin- and NADPH oxidase-mediated defense responses.
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DOI：
10.1371/journal.pone.0014426
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发表时间：
2010-12-23
期刊：
影响因子：
3.7
通讯作者：
Somerville S
中科院分区：
文献类型：
--
作者：
Berrocal-Lobo M;Stone S;Yang X;Antico J;Callis J;Ramonell KM;Somerville S
病原菌相关分子模式(PAMP)是植物检测到的激活基础防御的信号。其中一种PAMP是几丁质，一种存在于真菌细胞壁和昆虫外骨骼中的碳水化合物。前人的工作表明，甲壳素处理拟南芥在没有病原菌的情况下诱导了与防御相关的基因，并且这种反应不依赖于水杨酸(SA)、茉莉酸(JA)和乙烯(ET)信号通路。其中一个基因是ATL9( = ATL2G)，它编码一种环状锌指蛋白。在目前的工作中，我们证明了ATL9具有E3泛素连接酶活性，并定位于内质网。ATL9的表达模式与其对黄腐菌的基本防御反应呈正相关。几丁质在野生型植物中的基础表达水平和对ATL9的诱导依赖于NADPH氧化酶的活性，这表明几丁质介导的防御反应是NADPH氧化酶依赖的。虽然已知的防御激素(SA、JA或ET)处理不能诱导ATL9的表达，但在ET或SA依赖的信号被抑制的突变体中，对甲壳素的完全响应受到轻微的影响。对atl9突变体的微阵列分析显示，在甲壳素介导的防御中，候选基因似乎作用于atl9下游。这些结果暗示了甲壳素介导的信号转导的复杂性，以及激发子介导的信号转导、已知防御途径的信号转导和氧化爆发之间的潜在相互作用。
Pathogen associated molecular patterns (PAMPs) are signals detected by plants that activate basal defenses. One of these PAMPs is chitin, a carbohydrate present in the cell walls of fungi and in insect exoskeletons. Previous work has shown that chitin treatment of Arabidopsis thaliana induced defense-related genes in the absence of a pathogen and that the response was independent of the salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) signaling pathways. One of these genes is ATL9 ( = ATL2G), which encodes a RING zinc-finger like protein. In the current work we demonstrate that ATL9 has E3 ubiquitin ligase activity and is localized to the endoplasmic reticulum. The expression pattern of ATL9 is positively correlated with basal defense responses against Golovinomyces cichoracearum, a biotrophic fungal pathogen. The basal levels of expression and the induction of ATL9 by chitin, in wild type plants, depends on the activity of NADPH oxidases suggesting that chitin-mediated defense response is NADPH oxidase dependent. Although ATL9 expression is not induced by treatment with known defense hormones (SA, JA or ET), full expression in response to chitin is compromised slightly in mutants where ET- or SA-dependent signaling is suppressed. Microarray analysis of the atl9 mutant revealed candidate genes that appear to act downstream of ATL9 in chitin-mediated defenses. These results hint at the complexity of chitin-mediated signaling and the potential interplay between elicitor-mediated signaling, signaling via known defense pathways and the oxidative burst.
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影响因子：
6.9
作者：
Mukoko Bopopi J;Vandeputte OM;Himanen K;Mol A;Vaessen Q;El Jaziri M;Baucher M
通讯作者：
Baucher M
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
DOI：
10.1073/pnas.97.7.3718
发表时间：
2000-03-28
影响因子：
11.1
作者：
Cutler, SR;Ehrhardt, DW;Somerville, CR
通讯作者：
Somerville, CR
影响因子：
7.2
作者：
Berrocal-Lobo, M;Molina, A;Solano, R
通讯作者：
Solano, R
影响因子：
11.6
作者：
Durrant, WE;Rowland, O;Jones, JDG
通讯作者：
Jones, JDG