Ectopic expression of PtaRHE1, encoding a poplar RING-H2 protein with E3 ligase activity, alters plant development and induces defence-related responses.
Ectopic expression of PtaRHE1, encoding a poplar RING-H2 protein with E3 ligase activity, alters plant development and induces defence-related responses.
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DOI：
10.1093/jxb/erp305
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发表时间：
2010
影响因子：
6.9
通讯作者：
Baucher M
中科院分区：
文献类型：
--
作者：
Mukoko Bopopi J;Vandeputte OM;Himanen K;Mol A;Vaessen Q;El Jaziri M;Baucher M
RING(真正有趣的新基因)-H2结构域蛋白在植物中广泛存在，在许多发育过程的调控以及植物与环境的相互作用中发挥着重要作用。在本报告中，我们进行了实验来解开杨树基因PtaRHE1的作用，PtaRHE1编码一个环-H2蛋白。体外泛素化试验表明，PtaRHE1与特定的E2泛素结合酶UbcH5a具有功能性的E3连接酶活性。PtaRHE1在烟草中的过表达导致了一种多效性表型，其特征是叶片卷曲，叶片上形成坏死斑，生长迟缓，花朵转换延迟。植物对PtaRHE1过表达的基因表达反应为防御和/或程序性细胞死亡相关基因的上调提供了证据。此外，与野生型相比，编码WRKY转录因子和有丝分裂原激活的蛋白激酶(如WIPK)的基因也在转基因株系中被诱导。此外，组织化学β-葡萄糖醛酸苷酶染色表明，PtaRHE1启动子受植物病原菌以及水杨酸和纤维素酶等激发子的诱导。综上所述，这些结果表明，E3连接酶PtaRHE1可能通过作用于WIPK上游和/或激活WRKY因子，在泛素化介导的防御反应调节中发挥作用。
RING (really interesting new gene)-H2 domain-containing proteins are widely represented in plants and play important roles in the regulation of many developmental processes as well as in plant–environment interactions. In the present report, experiments were performed to unravel the role of the poplar gene PtaRHE1, coding for a RING-H2 protein. In vitro ubiquitination assays indicate a functional E3 ligase activity for PtaRHE1 with the specific E2 ubiquitin-conjugating enzyme UbcH5a. The overexpression of PtaRHE1 in tobacco resulted in a pleiotropic phenotype characterized by a curling of the leaves, the formation of necrotic lesions on leaf blades, growth retardation, and a delay in floral transition. The plant gene expression response to PtaRHE1 overexpression provided evidence for the up-regulation of defence- and/or programmed cell death-related genes. Moreover, genes coding for WRKY transcription factors as well as for mitogen-activated protein kinases, such as wound-induced protein kinase (WIPK), were also found to be induced in the transgenic lines as compared with the wild type. In addition, histochemical β-glucuronidase staining showed that the PtaRHE1 promoter is induced by plant pathogens and by elicitors such as salicylic acid and cellulase. Taken together, these results suggest that the E3 ligase PtaRHE1 plays a role in the ubiquitination-mediated regulation of defence response, possibly by acting upstream of WIPK and/or in the activation of WRKY factors.
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影响因子：
4.8
作者：
Katoh, S;Tsunoda, Y;Katoh, E
通讯作者：
Katoh, E
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